{"id":"33e608c0-1074-5d2d-a9b2-bddef83f3222","stable_key":"3ad23a58-d6a8-5554-81d6-9c83abd33087:gpx1-insulin-signaling-counterevidence","predicate":"reported_comparison","statement":"GPX1-overexpressing mice showed reduced insulin-stimulated receptor phosphorylation in liver and Akt phosphorylation in liver and soleus.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ec09d415-1dfb-53c1-bf0b-98b9d21cb297","mechanism_event_label":"GPX1-overexpressing mice showed reduced insulin-stimulated receptor phosphorylation in liver and Akt phosphorylation in liver and soleus.","subject":{"id":"20f3e45f-87e7-53b5-aea5-d318307f2a36","slug":"mouse-gpx1-overexpression","display_name":"Mouse Gpx1 overexpression genotype","entity_type_key":"gene"},"object":{"id":"de559400-6a45-5ef3-80e3-ebe96ef4b83a","slug":"mouse-insr","display_name":"Mouse insulin receptor / Insr","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ec09d415-1dfb-53c1-bf0b-98b9d21cb297","stable_key":"3ad23a58-d6a8-5554-81d6-9c83abd33087:gpx1-insulin-signaling-counterevidence","event_type":"reported_comparison","label":"GPX1-overexpressing mice showed reduced insulin-stimulated receptor phosphorylation in liver and Akt phosphorylation in liver and soleus.","description":"","status":"provisional","compartment":null,"participants":[{"entity":{"id":"20f3e45f-87e7-53b5-aea5-d318307f2a36","slug":"mouse-gpx1-overexpression","display_name":"Mouse Gpx1 overexpression genotype","entity_type_key":"gene"},"role":"experimental_actor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"de559400-6a45-5ef3-80e3-ebe96ef4b83a","slug":"mouse-insr","display_name":"Mouse insulin receptor / Insr","entity_type_key":"protein"},"role":"measured_endpoint","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8f9a788e-0c0b-5b9a-90cb-3b71bb2eea31","slug":"mouse-gpx1","display_name":"Mouse glutathione peroxidase 1 / Gpx1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"43dee7e1-274c-533f-89ea-c91521728a8f","slug":"mouse-akt-proteins","display_name":"Mouse Akt protein family (isoform unresolved)","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"dc89d7e2-8f89-577b-838f-42bf5d8ec7c8","slug":"selenium","display_name":"Selenium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"curation_topic","value_text":"selenium","comparator":null,"unit":null,"notes":"","entity":{"slug":"selenium","display_name":"Selenium","entity_type_key":"nutrient_element"}},{"dimension":"experimental_condition","value_text":"GPX1 overexpression","comparator":"Wild-type mice on the same diet","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"mouse-gpx1-overexpression","display_name":"Mouse Gpx1 overexpression genotype","entity_type_key":"gene"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"GPX1 overexpression\", \"comparator\": \"Wild-type mice on the same diet\", \"endpoint\": \"Insulin-stimulated phosphorylation\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": [{\"entity_slug\": \"mouse-gpx1-overexpression\", \"state\": \"GPX1 overexpression\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Male transgenic mice; selenium-adequate 0.4 mg/kg diet, ages 8 to 24 weeks","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Overexpression experiment, not dietary selenium excess. Excess ROS quenching is a proposed explanation rather than a measured universal mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"McClung et al. 2004; DOI:10.1073/pnas.0308096101; PMID:15184668; https://pmc.ncbi.nlm.nih.gov/articles/PMC428436/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c149ddd6-4726-53a1-860d-15617b3c9ef0","evidence_kind":"source_excerpt","locator":"Lines 30-30","start_line":30,"end_line":30,"excerpt":"GPX1-overexpressing mice showed reduced insulin-stimulated receptor phosphorylation in liver and Akt phosphorylation in liver and soleus. Model: Male transgenic mice; selenium-adequate 0.4 mg/kg diet, ages 8 to 24 weeks. Limits: Overexpression experiment, not dietary selenium excess. Excess ROS quenching is a proposed explanation rather than a measured universal mechanism. Primary reference: McClung et al. 2004; DOI:10.1073/pnas.0308096101; PMID:15184668; https://pmc.ncbi.nlm.nih.gov/articles/PMC428436/","model_system":"Male transgenic mice; selenium-adequate 0.4 mg/kg diet, ages 8 to 24 weeks","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curator paraphrase of the cited primary result; see stated access scope.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"8600b90c-477e-569e-9666-09321af7bc9e","stable_key":"import-3ad23a58-d6a8-5554-81d6-9c83abd33087","title":"Diabetes cascade: targeted primary-source supplement","document_type":"imported_text","citation_label":"See claim-local references; curated paraphrases reviewed 2026-09-20.","file_path":"","sha256":"0a841d67ea40a5efea21157e1a5703488b8ef63e587eea50dacac19c67894cd4","revision_id":"0faa1a78-e820-559e-a23b-5e3939f9079e","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}