{"id":"33cc0cee-d422-5ee5-92e4-0d6b4338d6a4","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-degradation-product-resistance","predicate":"poorly_hydrolyzes_tested","statement":"Cyclo-Asp-Phe and beta-Asp-Phe methyl ester resisted the tested microvillar and purified A/W peptidase preparations.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"c2577719-068a-5b2a-9820-99ad9510e892","mechanism_event_label":"Changed molecular geometry changes processing.","subject":{"id":"164d6ac3-0038-5071-b0dc-d6774ef52141","slug":"human-intestinal-aspartame-hydrolysis","display_name":"Human intestinal microvillar aspartame hydrolysis","entity_type_key":"cellular_process"},"object":{"id":"93a48846-8439-561e-9347-737591d2e27d","slug":"aspartame-diketopiperazine","display_name":"Aspartame diketopiperazine / cyclo-Asp-Phe","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"c2577719-068a-5b2a-9820-99ad9510e892","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-degradation-product-resistance-event","event_type":"observed_relationship","label":"Changed molecular geometry changes processing.","description":"Cyclo-Asp-Phe and beta-Asp-Phe methyl ester resisted the tested microvillar and purified A/W peptidase preparations.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"164d6ac3-0038-5071-b0dc-d6774ef52141","slug":"human-intestinal-aspartame-hydrolysis","display_name":"Human intestinal microvillar aspartame hydrolysis","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"93a48846-8439-561e-9347-737591d2e27d","slug":"aspartame-diketopiperazine","display_name":"Aspartame diketopiperazine / cyclo-Asp-Phe","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bd76f64b-bd0a-5b43-8e60-93d05f4c5d47","slug":"beta-aspartyl-phenylalanine-methyl-ester","display_name":"Beta-aspartyl-phenylalanine methyl ester","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human/pig membranes and purified peptidases.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Enzyme resistance does not establish toxicity or systemic persistence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Changed molecular geometry changes processing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolism of aspartame by human and pig intestinal microvillar peptidases. · 1994 · https://pubmed.ncbi.nlm.nih.gov/8141778/ · DOI 10.1042/bj2980635","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5c82f5f6-dedf-544b-bcf1-5f9e8c93a00b","evidence_kind":"source_excerpt","locator":"Lines 74-80","start_line":74,"end_line":80,"excerpt":"## aspartame-degradation-product-resistance\nChanged molecular geometry changes processing.\nCyclo-Asp-Phe and beta-Asp-Phe methyl ester resisted the tested microvillar and purified A/W peptidase preparations.\nModel: Human/pig membranes and purified peptidases.\nLimitations: Enzyme resistance does not establish toxicity or systemic persistence.\nEvidence access: Primary abstract\nMetabolism of aspartame by human and pig intestinal microvillar peptidases. · 1994 · https://pubmed.ncbi.nlm.nih.gov/8141778/ · DOI 10.1042/bj2980635","model_system":"Human/pig membranes and purified peptidases.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"21cfc6808062c6ea1fb5d34128bcd005d31f0f6c0d63484794238db77d32ae97","revision_id":"1255dda8-df99-5d4b-a65b-4144c47d7d9b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}