{"id":"33bf4af2-c794-53b8-8218-3d9968539b59","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-platelet-pathways","predicate":"inhibits","statement":"Eugenol suppressed collagen-induced PLC-gamma2/PKC, PI3K/Akt/GSK3beta, MAPK and cPLA2/thromboxane-A2 signaling in human platelets.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"d0d6d2b1-1ad7-55db-a44a-ccbb71751d9f","mechanism_event_label":"Eugenol suppressed collagen-induced PLC-gamma2/PKC, PI3K/Akt/GSK3beta, MAPK and cPLA2/thromboxane-A2 signaling in human platelets.","subject":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"object":{"id":"ece5fd35-9615-5ce8-a989-12c0c4c9c854","slug":"human-platelet-plcg2-pkc-cpla2-txa2-signaling","display_name":"Human platelet PLC-gamma2/PKC and cPLA2/thromboxane-A2 signaling","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d0d6d2b1-1ad7-55db-a44a-ccbb71751d9f","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-platelet-pathways-event","event_type":"observed_relationship","label":"Eugenol suppressed collagen-induced PLC-gamma2/PKC, PI3K/Akt/GSK3beta, MAPK and cPLA2/thromboxane-A2 signaling in human platelets.","description":"Eugenol suppressed collagen-induced PLC-gamma2/PKC, PI3K/Akt/GSK3beta, MAPK and cPLA2/thromboxane-A2 signaling in human platelets.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ece5fd35-9615-5ce8-a989-12c0c4c9c854","slug":"human-platelet-plcg2-pkc-cpla2-txa2-signaling","display_name":"Human platelet PLC-gamma2/PKC and cPLA2/thromboxane-A2 signaling","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"359979da-c958-50bf-b2f7-9f15e177fa5d","slug":"pi3k-family","display_name":"Phosphoinositide 3-kinase family","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a13cb592-c939-5f92-9206-d774248802fc","slug":"akt-proteins","display_name":"AKT serine/threonine kinase family","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Eugenol around the effective 2-micromolar range","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Washed human platelets","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pathway suppression does not identify one direct binding target and must not be generalized to every platelet agonist.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Eugenol chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Washed human platelets","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Eugenol suppressed collagen-induced PLC-gamma2/PKC, PI3K/Akt/GSK3beta, MAPK and cPLA2/thromboxane-A2 signaling in human platelets.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Eugenol Suppresses Platelet Activation and Mitigates Pulmonary Thromboembolism in Humans and Murine Models. (2024). https://pubmed.ncbi.nlm.nih.gov/38396774/ DOI: 10.3390/ijms25042098","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Signal phosphorylation, ATP release, P-selectin and calcium","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f734c761-57bb-50a7-aea7-4467b6fdddef","evidence_kind":"source_excerpt","locator":"Lines 22-31","start_line":22,"end_line":31,"excerpt":"## eugenol-platelet-pathways\nEugenol suppressed collagen-induced PLC-gamma2/PKC, PI3K/Akt/GSK3beta, MAPK and cPLA2/thromboxane-A2 signaling in human platelets.\nModel/species: Washed human platelets\nTissue/system: Signal phosphorylation, ATP release, P-selectin and calcium\nExposure: Eugenol around the effective 2-micromolar range\nRoute: In vitro\nDuration: Acute\nLimits: Pathway suppression does not identify one direct binding target and must not be generalized to every platelet agonist.\nPrimary reference: Eugenol Suppresses Platelet Activation and Mitigates Pulmonary Thromboembolism in Humans and Murine Models. (2024). https://pubmed.ncbi.nlm.nih.gov/38396774/ DOI: 10.3390/ijms25042098\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Washed human platelets","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f2ff5a4-7264-57fc-8368-46b9768ec668","stable_key":"import-2f309d3a-d252-5dfb-ae24-993a625f1b6c","title":"Eugenol: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"28c421c41a2fbfb00f3036084d43389247cfbfbad8f7549b7529fb4f0e84f61d","revision_id":"3377bcaa-7d21-522a-8571-50ff1977aba0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}