{"id":"339b94f4-1712-5ed2-bcbf-f18a91851010","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-flad1-isoform2-compartment","predicate":"localizes-to","statement":"FADS2 lacked mitochondrial import/localization in the experiments that imported and localized FADS1.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"cacbc176-2841-5c6e-9952-c065a59c8659","mechanism_event_label":"FLAD1 isoforms differ in access to the mitochondrial compartment.","subject":{"id":"992093d2-2665-50b6-8681-95705f09322b","slug":"flad1-isoform-2","display_name":"Human FAD synthetase isoform 2","entity_type_key":"protein"},"object":{"id":"bc9ed3a1-38d5-5bc3-b2a5-9f9f7633660b","slug":"cytosol","display_name":"Cytosol","entity_type_key":"organelle"},"evidence_count":1,"mechanism_event":{"id":"cacbc176-2841-5c6e-9952-c065a59c8659","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-flad1-isoform2-compartment-event","event_type":"biochemical_relationship","label":"FLAD1 isoforms differ in access to the mitochondrial compartment.","description":"FADS2 lacked mitochondrial import/localization in the experiments that imported and localized FADS1.","status":"provisional","compartment":{"slug":"cytosol","display_name":"Cytosol"},"participants":[{"entity":{"id":"992093d2-2665-50b6-8681-95705f09322b","slug":"flad1-isoform-2","display_name":"Human FAD synthetase isoform 2","entity_type_key":"protein"},"role":"nonimported-protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b872ce95-e881-5fcb-be38-8d8f8b0156e8","slug":"flad1-isoform-1","display_name":"Human FAD synthetase isoform 1","entity_type_key":"protein"},"role":"imported-comparator","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bc9ed3a1-38d5-5bc3-b2a5-9f9f7633660b","slug":"cytosol","display_name":"Cytosol","entity_type_key":"organelle"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_location","value_text":"Abstract; FADS2 comparator","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Parallel FADS1/FADS2 import and localization comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"In vitro synthesized and transiently expressed human isoforms.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No import in this assay does not exclude condition-dependent localization outside the tested systems.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human proteins in mammalian model preparations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"FLAD1 isoforms differ in access to the mitochondrial compartment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[transport-fads-localization-2010] Mitochondrial localization of human FAD synthetase isoform 1. (2010). https://pubmed.ncbi.nlm.nih.gov/20060505/ DOI: 10.1016/j.mito.2009.12.149","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Rat liver mitochondria; BHK-21 and Caco-2 cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4fff801a-439e-56db-b177-98128a29336c","evidence_kind":"source_excerpt","locator":"Lines 360-371","start_line":360,"end_line":371,"excerpt":"### transport-flad1-isoform2-compartment\nFADS2 lacked mitochondrial import/localization in the experiments that imported and localized FADS1.\nCondition category: normal\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: FLAD1 isoforms differ in access to the mitochondrial compartment.\norganism: Human proteins in mammalian model preparations\ntissue_or_cell_type: Rat liver mitochondria; BHK-21 and Caco-2 cells\nexperimental_model: Parallel FADS1/FADS2 import and localization comparison\nlimitations: No import in this assay does not exclude condition-dependent localization outside the tested systems.\nexposure: In vitro synthesized and transiently expressed human isoforms.\nevidence_location: Abstract; FADS2 comparator\n[transport-fads-localization-2010] Mitochondrial localization of human FAD synthetase isoform 1. 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