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High-affinity radioligand binding is separated from the sheep functional assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Melatonin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"melatonin","display_name":"Melatonin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human and sheep MT1 in cells; functional cyclase assay used sheep receptor","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The receptor connects to an inhibitory signaling system.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[melatonin-p7946354] Cloning and characterization of a mammalian melatonin receptor that mediates reproductive and circadian responses. 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High-affinity radioligand binding is separated from the sheep functional assay.\nexposure: Heterologous COS-7 binding and NIH3T3 functional assays\nevidence_span: {\"source_cache\": \"artifacts/melatonin-research/7946354.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"4eed36768519d1c02ed67434eb3dc08eb561de445d3532113e7eaeb2827e021f\", \"start_char\": 0, \"end_char\": 1054, \"text_sha256\": \"4eed36768519d1c02ed67434eb3dc08eb561de445d3532113e7eaeb2827e021f\"}\n[melatonin-p7946354] Cloning and characterization of a mammalian melatonin receptor that mediates reproductive and circadian responses. (1994). https://pubmed.ncbi.nlm.nih.gov/7946354/ DOI: 10.1016/0896-6273(94)90055-8","model_system":"Receptor cloning, radioligand binding and functional expression","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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