{"id":"3391544d-f450-55dd-9201-af7aa3108e8b","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-tg-geometry","predicate":"depends_on","statement":"Mutational and engineered-scaffold experiments implicated tyrosine proximity, flexibility and solvent exposure in hormone formation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"9aca66d9-0720-5e52-abf3-2961a2a1530b","mechanism_event_label":"Having the building blocks is not enough; their arrangement matters.","subject":{"id":"7d332c8d-cb33-5dcc-bbfe-0629c4118f5d","slug":"human-tg-hormonogenic-tyrosine-pairs","display_name":"Human thyroglobulin hormonogenic tyrosine pairs","entity_type_key":"cellular_process"},"object":{"id":"f3b8a45f-df71-57b6-b649-f7b75624e556","slug":"human-tg-site-accessibility","display_name":"Human thyroglobulin tyrosine proximity, flexibility and solvent exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9aca66d9-0720-5e52-abf3-2961a2a1530b","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-tg-geometry-event","event_type":"observed_relationship","label":"Having the building blocks is not enough; 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species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Having the building blocks is not enough; their arrangement matters.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The structure of human thyroglobulin. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32025030/ · DOI 10.1038/s41586-020-1995-4","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e985e1b4-1e87-5bbb-97f2-efd3b18a3a27","evidence_kind":"source_excerpt","locator":"Lines 140-146","start_line":140,"end_line":146,"excerpt":"## l-tyrosine-tg-geometry\nHaving the building blocks is not enough; their arrangement matters.\nMutational and engineered-scaffold experiments implicated tyrosine proximity, flexibility and solvent exposure in hormone formation.\nModel: Human TG and engineered bacterial maltose-binding-protein hormone-production assays.\nLimitations: The engineered bacterial scaffold is an in vitro demonstration, not a physiological human alternative.\nEvidence access: Primary abstract\nThe structure of human thyroglobulin. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32025030/ · DOI 10.1038/s41586-020-1995-4","model_system":"Human TG and engineered bacterial maltose-binding-protein hormone-production assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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