{"id":"332eada0-38b0-508d-a39e-6d04ecf5cad6","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-plasma-fad-nonresponse","predicate":"did_not_significantly_change_plasma_fad","statement":"Plasma FAD did not respond significantly to supplementation in the older-adult trial although plasma riboflavin, erythrocyte FMN and other measures did.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"033285aa-dbcb-5cfc-823d-7d97435e4059","mechanism_event_label":"A stable plasma FAD reading did not mean that all B2-related pools were unchanged.","subject":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"object":{"id":"463817b6-5215-5439-8915-5f299d3a41da","slug":"plasma-fad-concentration","display_name":"Plasma FAD concentration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"033285aa-dbcb-5cfc-823d-7d97435e4059","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-plasma-fad-nonresponse-event","event_type":"observed_intervention","label":"A stable plasma FAD reading did not mean that all B2-related pools were unchanged.","description":"Plasma FAD did not respond significantly to supplementation in the older-adult trial although plasma riboflavin, erythrocyte FMN and other measures did.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e2cd7179-f218-54e8-9ce9-7a836ae35fac","slug":"fad","display_name":"FAD","entity_type_key":"small_molecule"},"role":"measured_cofactor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"463817b6-5215-5439-8915-5f299d3a41da","slug":"plasma-fad-concentration","display_name":"Plasma FAD concentration","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"124 healthy older adults, mean age 69; 46 with EGRAC >=1.20 randomized equally to riboflavin or placebo.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"1.6 mg/day riboflavin versus placebo for 12 weeks; HPLC plasma/red-cell vitamer measurements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A result in this population does not invalidate every use of plasma FAD; cellular compartment and clinical state matter.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A stable plasma FAD reading did not mean that all B2-related pools were unchanged.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b2-hustad2002] Riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in human plasma and erythrocytes at baseline and after low-dose riboflavin supplementation (2002). https://pubmed.ncbi.nlm.nih.gov/12194936/ DOI: 10.1093/clinchem/48.9.1571","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human clinical setting","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"4a010bc4-a0d7-5ace-b5eb-5e5b27dcbdb3","evidence_kind":"source_excerpt","locator":"Lines 1629-1639","start_line":1629,"end_line":1639,"excerpt":"### b2-plasma-fad-nonresponse\nPlasma FAD did not respond significantly to supplementation in the older-adult trial although plasma riboflavin, erythrocyte FMN and other measures did.\nCondition category: biomarker_context\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A stable plasma FAD reading did not mean that all B2-related pools were unchanged.\norganism: Homo sapiens\ntissue_or_cell_type: Human clinical setting\nexperimental_model: 124 healthy older adults, mean age 69; 46 with EGRAC >=1.20 randomized equally to riboflavin or placebo.\nlimitations: A result in this population does not invalidate every use of plasma FAD; cellular compartment and clinical state matter.\nexposure: 1.6 mg/day riboflavin versus placebo for 12 weeks; HPLC plasma/red-cell vitamer measurements.\n[b2-hustad2002] Riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in human plasma and erythrocytes at baseline and after low-dose riboflavin supplementation (2002). https://pubmed.ncbi.nlm.nih.gov/12194936/ DOI: 10.1093/clinchem/48.9.1571","model_system":"124 healthy older adults, mean age 69; 46 with EGRAC >=1.20 randomized equally to riboflavin or placebo.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b2-hustad2002] Riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in human plasma and erythrocytes at baseline and after low-dose riboflavin supplementation (2002). https://pubmed.ncbi.nlm.nih.gov/12194936/ DOI: 10.1093/clinchem/48.9.1571","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"680cb6bc8249877f2410f551807f10d390fdd719014137d7414ba3420e29228d","revision_id":"7a61e299-908d-5372-860b-99ed190f9d7a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}