{"id":"328100fd-5d38-523f-b338-27dd22ee1943","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-glyr-opening","predicate":"activates","statement":"Glycine opened recombinant human alpha1 homomeric glycine receptors in HEK293 cells; rapid-application and single-channel assays resolved activation kinetics.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d8ea37e2-079a-5a19-89f6-d6307d523197","mechanism_event_label":"Glycine can directly open a receptor channel.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"b40b5391-68b1-5939-86b2-fa3b7e7681d8","slug":"human-glra1-homomer","display_name":"Human alpha1 homomeric glycine receptor","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"d8ea37e2-079a-5a19-89f6-d6307d523197","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-glyr-opening-event","event_type":"observed_relationship","label":"Glycine can directly open a receptor channel.","description":"Glycine opened recombinant human alpha1 homomeric glycine receptors in HEK293 cells; rapid-application and single-channel assays resolved activation kinetics.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b40b5391-68b1-5939-86b2-fa3b7e7681d8","slug":"human-glra1-homomer","display_name":"Human alpha1 homomeric glycine receptor","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human alpha1 receptor, rapid agonist application and patch recording.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Homomeric experimental receptor differs from native heteromeric receptor mixtures; chloride gradient determines current consequences.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Glycine can directly open a receptor channel.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Kinetic determinants of agonist action at the recombinant human glycine receptor. · 2003 · https://pubmed.ncbi.nlm.nih.gov/12679369/ · DOI 10.1113/jphysiol.2002.037796","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"16a6a18b-168e-5f08-b7e4-d4b8d535ecd1","evidence_kind":"source_excerpt","locator":"Lines 194-200","start_line":194,"end_line":200,"excerpt":"## glycine-glyr-opening\nGlycine can directly open a receptor channel.\nGlycine opened recombinant human alpha1 homomeric glycine receptors in HEK293 cells; rapid-application and single-channel assays resolved activation kinetics.\nModel: Human alpha1 receptor, rapid agonist application and patch recording.\nLimitations: Homomeric experimental receptor differs from native heteromeric receptor mixtures; chloride gradient determines current consequences.\nEvidence access: Primary abstract\nKinetic determinants of agonist action at the recombinant human glycine receptor. · 2003 · https://pubmed.ncbi.nlm.nih.gov/12679369/ · DOI 10.1113/jphysiol.2002.037796","model_system":"Human alpha1 receptor, rapid agonist application and patch recording.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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