{"id":"3234da74-71ad-5c4e-91c3-771bb4d975c3","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-serum-toxicity-rescue","predicate":"prevents","statement":"Aminoguanidine prevented spermidine-associated cytotoxicity in the serum-containing NS1-cell experiment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"decae5b6-17b7-5da9-9213-0a7b37751891","mechanism_event_label":"Blocking extracellular oxidation changed the result.","subject":{"id":"72265412-71f4-5ac6-85e2-cedc03fde294","slug":"aminoguanidine","display_name":"Aminoguanidine","entity_type_key":"small_molecule"},"object":{"id":"3f69fc54-a4ad-5134-9bc4-f5c6e6df797c","slug":"ns1-serum-spermidine-cytotoxicity","display_name":"NS1-cell serum-dependent spermidine cytotoxicity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"decae5b6-17b7-5da9-9213-0a7b37751891","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-serum-toxicity-rescue-event","event_type":"observed_relationship","label":"Blocking extracellular oxidation changed the result.","description":"Aminoguanidine prevented spermidine-associated cytotoxicity in the serum-containing NS1-cell experiment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"72265412-71f4-5ac6-85e2-cedc03fde294","slug":"aminoguanidine","display_name":"Aminoguanidine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3f69fc54-a4ad-5134-9bc4-f5c6e6df797c","slug":"ns1-serum-spermidine-cytotoxicity","display_name":"NS1-cell serum-dependent spermidine cytotoxicity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"57ee1063-a345-52f5-96ce-2be6b94912ae","slug":"serum-polyamine-oxidation-in-culture","display_name":"Serum-mediated polyamine oxidation in cell culture","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"1984 serum and oxygen-tension comparison; micromolar exposure range.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Historical serum oxidase activity is not automatically modern human SMOX identity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Blocking extracellular oxidation changed the result.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Spermidine cytotoxicity in vitro: effect of serum and oxygen tension. · 1984 · https://pubmed.ncbi.nlm.nih.gov/6715006/ · DOI 10.1007/BF02618188","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cefbb7ae-96da-54cc-82cb-4feb70cb82fa","evidence_kind":"source_excerpt","locator":"Lines 494-500","start_line":494,"end_line":500,"excerpt":"## spermidine-serum-toxicity-rescue\nBlocking extracellular oxidation changed the result.\nAminoguanidine prevented spermidine-associated cytotoxicity in the serum-containing NS1-cell experiment.\nModel: 1984 serum and oxygen-tension comparison; micromolar exposure range.\nLimitations: Historical serum oxidase activity is not automatically modern human SMOX identity.\nEvidence access: Primary abstract\nSpermidine cytotoxicity in vitro: effect of serum and oxygen tension. · 1984 · https://pubmed.ncbi.nlm.nih.gov/6715006/ · DOI 10.1007/BF02618188","model_system":"1984 serum and oxygen-tension comparison; micromolar exposure range.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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