{"id":"322eb1db-a090-535f-b955-e78f0d6ecf05","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-met-complex-i-flavin-specificity","predicate":"does-not-directly-reconstitute","statement":"Riboflavin and FAD failed to protect or reactivate alkaline-inactivated bovine complex I under conditions where FMN did.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"94b7183e-5ac0-5ade-91a4-c493ab4cf5b4","mechanism_event_label":"Free B2 and FAD could not substitute directly for FMN in this test tube.","subject":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"object":{"id":"f67ac698-de2e-55f5-8975-305eaadbb751","slug":"respiratory-complex-i","display_name":"Mitochondrial respiratory complex I","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"94b7183e-5ac0-5ade-91a4-c493ab4cf5b4","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-met-complex-i-flavin-specificity-event","event_type":"biochemical_relationship","label":"Free B2 and FAD could not substitute directly for FMN in this test tube.","description":"Riboflavin and FAD failed to protect or reactivate alkaline-inactivated bovine complex I under conditions where FMN did.","status":"provisional","compartment":{"slug":"mitochondria","display_name":"Mitochondria"},"participants":[{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"ineffective direct replacement","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e2cd7179-f218-54e8-9ce9-7a836ae35fac","slug":"fad","display_name":"FAD","entity_type_key":"small_molecule"},"role":"ineffective direct replacement","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d3eacf85-1a35-546d-bd30-161474dd9691","slug":"fmn","display_name":"Flavin mononucleotide","entity_type_key":"small_molecule"},"role":"effective direct replacement","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f67ac698-de2e-55f5-8975-305eaadbb751","slug":"respiratory-complex-i","display_name":"Mitochondrial respiratory complex I","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_spans","value_text":"[{\"source_bundle\": \"artifacts/riboflavin_metabolism_sources.json\", \"source_key\": \"PMC2440658\", \"locator\": \"HTML article p\", \"paragraph_index\": 11, \"char_start\": 0, \"char_end\": 1107, \"evidence_access\": \"full-text\"}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Bovine heart submitochondrial particles; alkaline reductive inactivation and cofactor reconstitution.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"10 micromolar riboflavin, FAD or FMN in the reconstitution comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not test intact-cell conversion of riboflavin into FMN.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Bos taurus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Free B2 and FAD could not substitute directly for FMN in this test tube.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[gostimskaya-2007-complex-i-fmn] Reversible dissociation of flavin mononucleotide from the mammalian membrane-bound NADH: ubiquinone oxidoreductase (complex I) (2007). https://pubmed.ncbi.nlm.nih.gov/18037377/ DOI: 10.1016/j.febslet.2007.11.048","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Heart submitochondrial particles","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"f6fc067e-5b20-51cc-a557-8dc30afd2c87","evidence_kind":"source_excerpt","locator":"Lines 596-607","start_line":596,"end_line":607,"excerpt":"### b2-met-complex-i-flavin-specificity\nRiboflavin and FAD failed to protect or reactivate alkaline-inactivated bovine complex I under conditions where FMN did.\nCondition category: machinery_impairment\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Free B2 and FAD could not substitute directly for FMN in this test tube.\norganism: Bos taurus\ntissue_or_cell_type: Heart submitochondrial particles\nexperimental_model: Bovine heart submitochondrial particles; alkaline reductive inactivation and cofactor reconstitution.\nlimitations: Does not test intact-cell conversion of riboflavin into FMN.\nexposure: 10 micromolar riboflavin, FAD or FMN in the reconstitution comparison.\nevidence_spans: [{\"source_bundle\": \"artifacts/riboflavin_metabolism_sources.json\", \"source_key\": \"PMC2440658\", \"locator\": \"HTML article p\", \"paragraph_index\": 11, \"char_start\": 0, \"char_end\": 1107, \"evidence_access\": \"full-text\"}]\n[gostimskaya-2007-complex-i-fmn] Reversible dissociation of flavin mononucleotide from the mammalian membrane-bound NADH: ubiquinone oxidoreductase (complex I) (2007). https://pubmed.ncbi.nlm.nih.gov/18037377/ DOI: 10.1016/j.febslet.2007.11.048","model_system":"Bovine heart submitochondrial particles; alkaline reductive inactivation and cofactor reconstitution.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [gostimskaya-2007-complex-i-fmn] Reversible dissociation of flavin mononucleotide from the mammalian membrane-bound NADH: ubiquinone oxidoreductase (complex I) (2007). https://pubmed.ncbi.nlm.nih.gov/18037377/ DOI: 10.1016/j.febslet.2007.11.048","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"680cb6bc8249877f2410f551807f10d390fdd719014137d7414ba3420e29228d","revision_id":"7a61e299-908d-5372-860b-99ed190f9d7a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}