{"id":"32289111-6dce-55f8-9406-29b1b42fff7e","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:dhps-eif5a-deoxyhypusine","predicate":"aminobutylates","statement":"DHPS transfers spermidine-derived aminobutyl onto eIF5A Lys50 to form deoxyhypusine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a19937f4-4020-5433-b626-f060473653a3","mechanism_event_label":"A particular protein lysine becomes a specialized translation-factor residue.","subject":{"id":"33684e20-5166-55d6-9196-0793156787f1","slug":"dhps","display_name":"DHPS","entity_type_key":"protein"},"object":{"id":"2f597a76-0e80-51ce-bed0-4859d7ae2685","slug":"eif5a1-k50","display_name":"eIF5A1 with unmodified Lys50","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"a19937f4-4020-5433-b626-f060473653a3","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:dhps-eif5a-deoxyhypusine-event","event_type":"biochemical_relationship","label":"A particular protein lysine becomes a specialized translation-factor residue.","description":"DHPS transfers spermidine-derived aminobutyl onto eIF5A Lys50 to form deoxyhypusine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"33684e20-5166-55d6-9196-0793156787f1","slug":"dhps","display_name":"DHPS","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2f597a76-0e80-51ce-bed0-4859d7ae2685","slug":"eif5a1-k50","display_name":"eIF5A1 with unmodified Lys50","entity_type_key":"protein_state"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"donor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"catalytic_cofactor","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"a4d54591-b5a7-5e54-a52c-7011ace70819","slug":"eif5a1-deoxyhypusine","display_name":"eIF5A1 with deoxyhypusine at residue 50","entity_type_key":"protein_state"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"5120052e-17b6-5962-83aa-a365e4ae8a0c","slug":"1-3-diaminopropane","display_name":"1,3-Diaminopropane","entity_type_key":"small_molecule"},"role":"coproduct","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human DHPS structural biochemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"NAD cycles during catalysis; it is not represented as a net consumed substrate here. This reaction modifies lysine already in a protein. It does not show that extra oral lysine increases the reaction or improves a clinical outcome.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A particular protein lysine becomes a specialized translation-factor residue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[dhps-2020] Half Way to Hypusine-Structural Basis for Substrate Recognition by Human Deoxyhypusine Synthase (2020). https://pubmed.ncbi.nlm.nih.gov/32235505/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Not specified as a whole tissue; see experimental model.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ec0f531f-fe07-55d4-a930-a7c88ce87762","evidence_kind":"source_excerpt","locator":"Lines 578-586","start_line":578,"end_line":586,"excerpt":"### dhps-eif5a-deoxyhypusine\nDHPS transfers spermidine-derived aminobutyl onto eIF5A Lys50 to form deoxyhypusine.\nPlain language: A particular protein lysine becomes a specialized translation-factor residue.\nCondition category: normal\norganism: Human\ntissue_or_cell_type: Not specified as a whole tissue; see experimental model.\nexperimental_model: Human DHPS structural biochemistry.\nlimitations: NAD cycles during catalysis; it is not represented as a net consumed substrate here. This reaction modifies lysine already in a protein. It does not show that extra oral lysine increases the reaction or improves a clinical outcome.\n[dhps-2020] Half Way to Hypusine-Structural Basis for Substrate Recognition by Human Deoxyhypusine Synthase (2020). https://pubmed.ncbi.nlm.nih.gov/32235505/","model_system":"Human DHPS structural biochemistry.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [dhps-2020] Half Way to Hypusine-Structural Basis for Substrate Recognition by Human Deoxyhypusine Synthase (2020). https://pubmed.ncbi.nlm.nih.gov/32235505/","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}