{"id":"31c72ef5-1bda-5728-9b46-fc989468fd0c","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-polyglutamate-no-mito-export","predicate":"shows-no-detectable","statement":"Tracer experiments detected no intact folate-polyglutamate transfer from mitochondria to cytosol in the tested human-FPGS-reconstituted hamster cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"e4c9d71a-9e35-500e-a6ca-54264e69e94b","mechanism_event_label":"Stored mitochondrial folates did not measurably replenish the cytosol.","subject":{"id":"81c45abb-713d-521f-9854-23288f880831","slug":"folate-polyglutamates","display_name":"Folate polyglutamates","entity_type_key":"chemical_species"},"object":{"id":"e14c22b3-664b-5bfe-aaeb-3a3d3d512a45","slug":"mitochondrial-folate-polyglutamate-export","display_name":"Mitochondrial folate polyglutamate export","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e4c9d71a-9e35-500e-a6ca-54264e69e94b","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-polyglutamate-no-mito-export-event","event_type":"biochemical_relationship","label":"Stored mitochondrial folates did not measurably replenish the cytosol.","description":"Tracer experiments detected no intact folate-polyglutamate transfer from mitochondria to cytosol in the tested human-FPGS-reconstituted hamster cells.","status":"provisional","compartment":{"slug":"mitochondria","display_name":"Mitochondria"},"participants":[{"entity":{"id":"81c45abb-713d-521f-9854-23288f880831","slug":"folate-polyglutamates","display_name":"Folate polyglutamates","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e14c22b3-664b-5bfe-aaeb-3a3d3d512a45","slug":"mitochondrial-folate-polyglutamate-export","display_name":"Mitochondrial folate polyglutamate export","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human FPGS isoforms in Chinese hamster AUXB1 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Compartment-specific FPGS induction and tracer chase","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Detection-limited result, not proof of universal impermeability.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human enzyme in Cricetulus griseus cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Stored mitochondrial folates did not measurably replenish the cytosol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lawrence2014] Mammalian mitochondrial and cytosolic folylpolyglutamate synthetase maintain the subcellular compartmentalization of folates (2014). https://pubmed.ncbi.nlm.nih.gov/25164808/ DOI: 10.1074/jbc.m114.593244","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"AUXB1 mitochondrial and cytosolic fractions","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6f4aa584-3c02-58de-a7bf-2ad25630a3c8","evidence_kind":"source_excerpt","locator":"Lines 303-313","start_line":303,"end_line":313,"excerpt":"### folate-polyglutamate-no-mito-export\nTracer experiments detected no intact folate-polyglutamate transfer from mitochondria to cytosol in the tested human-FPGS-reconstituted hamster cells.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Stored mitochondrial folates did not measurably replenish the cytosol.\norganism: Human enzyme in Cricetulus griseus cells\ntissue_or_cell_type: AUXB1 mitochondrial and cytosolic fractions\nexperimental_model: Human FPGS isoforms in Chinese hamster AUXB1 cells\nlimitations: Detection-limited result, not proof of universal impermeability.\nexposure: Compartment-specific FPGS induction and tracer chase\n[lawrence2014] Mammalian mitochondrial and cytosolic folylpolyglutamate synthetase maintain the subcellular compartmentalization of folates (2014). https://pubmed.ncbi.nlm.nih.gov/25164808/ DOI: 10.1074/jbc.m114.593244","model_system":"Human FPGS isoforms in Chinese hamster AUXB1 cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [lawrence2014] Mammalian mitochondrial and cytosolic folylpolyglutamate synthetase maintain the subcellular compartmentalization of folates (2014). https://pubmed.ncbi.nlm.nih.gov/25164808/ DOI: 10.1074/jbc.m114.593244","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}