{"id":"31c6c986-09ed-5383-9dcc-fa7de388d0dd","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-neuronal-proteasome","predicate":"increases_activity","statement":"Fisetin increased proteasome activity and supported low-density rat cortical-neuron survival without trophic factors.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"25be016a-21d9-54c9-aef5-8194af953351","mechanism_event_label":"Protein turnover contributed to neuronal survival.","subject":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"object":{"id":"6996fb6e-548d-597b-8a6b-97e4b468da41","slug":"rat-neuronal-proteasome","display_name":"Rat cortical-neuron proteasome activity","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"25be016a-21d9-54c9-aef5-8194af953351","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-neuronal-proteasome-event","event_type":"observed_relationship","label":"Protein turnover contributed to neuronal survival.","description":"Fisetin increased proteasome activity and supported low-density rat cortical-neuron survival without trophic factors.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6996fb6e-548d-597b-8a6b-97e4b468da41","slug":"rat-neuronal-proteasome","display_name":"Rat cortical-neuron proteasome activity","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Serum-free rat neuron cultures.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The survival response was not dependent on glutathione or ERK in this experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Protein turnover contributed to neuronal survival.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The flavonoid fisetin promotes nerve cell survival from trophic factor withdrawal by enhancement of proteasome activity. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18396148/ · DOI 10.1016/j.abb.2008.03.023","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b1daf1fd-2fc9-506b-a592-997eba00a557","evidence_kind":"source_excerpt","locator":"Lines 352-358","start_line":352,"end_line":358,"excerpt":"## fisetin-neuronal-proteasome\nProtein turnover contributed to neuronal survival.\nFisetin increased proteasome activity and supported low-density rat cortical-neuron survival without trophic factors.\nModel: Serum-free rat neuron cultures.\nLimitations: The survival response was not dependent on glutathione or ERK in this experiment.\nEvidence access: Primary abstract\nThe flavonoid fisetin promotes nerve cell survival from trophic factor withdrawal by enhancement of proteasome activity. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18396148/ · DOI 10.1016/j.abb.2008.03.023","model_system":"Serum-free rat neuron cultures.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c30e0764cd34bd062cfcbfaf32357ef29dfda30e7d4153c545178140f5054e09","revision_id":"40ff52c0-f4b9-5820-8abb-ff002c6364a4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}