{"id":"319267cf-5c40-58d5-a711-fbf875ac1fe1","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-rbc-dha-recycling","predicate":"is-reduced-to","statement":"Human erythrocytes rapidly took up supplied DHA and accumulated ascorbate to concentrations up to 2 mM in the ex vivo assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"fe7ec59c-0b15-5975-969e-5d8621d3ea6b","mechanism_event_label":"Red cells can recover reduced vitamin C from its oxidized form.","subject":{"id":"e6658c2b-ccff-5122-80a5-2e153f12ba90","slug":"dehydroascorbic-acid","display_name":"Dehydroascorbic acid","entity_type_key":"small_molecule"},"object":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"fe7ec59c-0b15-5975-969e-5d8621d3ea6b","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-rbc-dha-recycling-event","event_type":"biochemical_relationship","label":"Red cells can recover reduced vitamin C from its oxidized form.","description":"Human erythrocytes rapidly took up supplied DHA and accumulated ascorbate to concentrations up to 2 mM in the ex vivo assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"reducing system","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e6658c2b-ccff-5122-80a5-2e153f12ba90","slug":"dehydroascorbic-acid","display_name":"Dehydroascorbic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human volunteer erythrocytes ex vivo","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Exogenous DHA concentration series","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay loading capacity is not the normal circulating erythrocyte concentration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin C research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-c","display_name":"Vitamin C","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Red cells can recover reduced vitamin C from its oxidized form.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[may2001] Mechanisms of ascorbic acid recycling in human erythrocytes. (2001). https://pubmed.ncbi.nlm.nih.gov/11687303/ DOI: 10.1016/s0304-4165(01)00188-x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Erythrocytes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a8e8f010-3c00-57dd-9784-7e7b79c4e572","evidence_kind":"source_excerpt","locator":"Lines 312-323","start_line":312,"end_line":323,"excerpt":"### vc-transport-rbc-dha-recycling\nHuman erythrocytes rapidly took up supplied DHA and accumulated ascorbate to concentrations up to 2 mM in the ex vivo assay.\nCondition category: normal\nnutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Red cells can recover reduced vitamin C from its oxidized form.\norganism: Homo sapiens\ntissue_or_cell_type: Erythrocytes\nexperimental_model: Human volunteer erythrocytes ex vivo\nlimitations: Assay loading capacity is not the normal circulating erythrocyte concentration.\nexposure: Exogenous DHA concentration series\ncross_nutrient: true\n[may2001] Mechanisms of ascorbic acid recycling in human erythrocytes. (2001). https://pubmed.ncbi.nlm.nih.gov/11687303/ DOI: 10.1016/s0304-4165(01)00188-x","model_system":"Human volunteer erythrocytes ex vivo","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [may2001] Mechanisms of ascorbic acid recycling in human erythrocytes. (2001). https://pubmed.ncbi.nlm.nih.gov/11687303/ DOI: 10.1016/s0304-4165(01)00188-x","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"fa811221-13bd-5c10-adc1-eaf097c7703c","stable_key":"import-cb568d28-484a-5c2e-9fcc-2d780358e514","title":"Vitamin C: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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