{"id":"31795979-53cf-5416-a8f7-1274f8f6affe","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-dim-hydroxylation","predicate":"forms_in_humans","statement":"Two monohydroxylated and one dihydroxylated DIM products appeared in human plasma and urine.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5faa09f8-7b5c-5125-950e-3248bcc0450f","mechanism_event_label":"The body changes DIM into additional molecules that need their own records.","subject":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"object":{"id":"70945460-ab50-5c8b-9a2e-ea593789bab3","slug":"dim-hydroxylated-metabolites","display_name":"Hydroxylated DIM metabolites, mixed analytical pool","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"5faa09f8-7b5c-5125-950e-3248bcc0450f","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-dim-hydroxylation-event","event_type":"observed_intervention","label":"The body changes DIM into additional molecules that need their own records.","description":"Two monohydroxylated and one dihydroxylated DIM products appeared in human plasma and urine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"70945460-ab50-5c8b-9a2e-ea593789bab3","slug":"dim-hydroxylated-metabolites","display_name":"Hydroxylated DIM metabolites, mixed analytical pool","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/dim-research/34035125.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0df37954db1da2ae488edb763e1397977b65d206cdd52269a5a882b62e8748ff\", \"start_char\": 0, \"end_char\": 2514, \"text_sha256\": \"0df37954db1da2ae488edb763e1397977b65d206cdd52269a5a882b62e8748ff\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human serial plasma/urine mass spectrometry and reporter assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Two BR-DIM 150 capsules nightly for one week; measured 45.3 mg DIM per capsule","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Small mixed-sex sample. Product label mass and measured DIM content differ. Some metabolite structures were tentative; reporter activity was not a clinical response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Seven adults; mouse Hepa1 reporter cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The body changes DIM into additional molecules that need their own records.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[dim-p34035125] 3,3'-Diindolylmethane Exhibits Significant Metabolism after Oral Dosing in Humans. (2021). https://pubmed.ncbi.nlm.nih.gov/34035125/ DOI: 10.1124/dmd.120.000346","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"DIM metabolism and metabolite signaling","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ed228f92-e705-5612-a622-ab93cdb9d81e","evidence_kind":"source_excerpt","locator":"Lines 207-218","start_line":207,"end_line":218,"excerpt":"### dim-dim-hydroxylation\nTwo monohydroxylated and one dihydroxylated DIM products appeared in human plasma and urine.\nCondition category: normal\nnutrient_topic: Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The body changes DIM into additional molecules that need their own records.\norganism: Seven adults; mouse Hepa1 reporter cells\ntissue_or_cell_type: DIM metabolism and metabolite signaling\nexperimental_model: Human serial plasma/urine mass spectrometry and reporter assays\nlimitations: Small mixed-sex sample. Product label mass and measured DIM content differ. Some metabolite structures were tentative; reporter activity was not a clinical response.\nexposure: Two BR-DIM 150 capsules nightly for one week; measured 45.3 mg DIM per capsule\nevidence_span: {\"source_cache\": \"artifacts/dim-research/34035125.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0df37954db1da2ae488edb763e1397977b65d206cdd52269a5a882b62e8748ff\", \"start_char\": 0, \"end_char\": 2514, \"text_sha256\": \"0df37954db1da2ae488edb763e1397977b65d206cdd52269a5a882b62e8748ff\"}\n[dim-p34035125] 3,3'-Diindolylmethane Exhibits Significant Metabolism after Oral Dosing in Humans. (2021). https://pubmed.ncbi.nlm.nih.gov/34035125/ DOI: 10.1124/dmd.120.000346","model_system":"Human serial plasma/urine mass spectrometry and reporter assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [dim-p34035125] 3,3'-Diindolylmethane Exhibits Significant Metabolism after Oral Dosing in Humans. (2021). https://pubmed.ncbi.nlm.nih.gov/34035125/ DOI: 10.1124/dmd.120.000346","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b6d70682-97f9-5893-a03c-f9f88836033c","stable_key":"import-182336c6-ed36-5ec6-8a09-25c31096262e","title":"Diindolylmethane (DIM): formation, receptor signaling, metabolism and drug interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"31e14060de904976d2f5d439f2bc9c8a99a72d34626c562c78429025e050d404","revision_id":"611094c1-a9cf-5b9b-b255-ac70b571696a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}