{"id":"3168c26d-9dbb-5ff1-930a-8af5df2a4872","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-trans-renal-zip14","predicate":"supports","statement":"ZIP14-specific siRNA reduced apical manganese uptake in polarized mouse proximal-tubule cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"33a01d19-b418-5768-9ce2-733cbd0913ae","mechanism_event_label":"ZIP14 helped kidney-derived cells take up manganese from their lumen-facing side.","subject":{"id":"9e0e70ec-aa5b-584d-a6ea-28c400cb3100","slug":"mouse-slc39a14","display_name":"Mouse ZIP14 (Slc39a14)","entity_type_key":"protein"},"object":{"id":"ef134340-d6ae-5c8f-8b76-303bcc66760a","slug":"cellular-manganese-uptake","display_name":"Cellular manganese uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"33a01d19-b418-5768-9ce2-733cbd0913ae","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-trans-renal-zip14-event","event_type":"biochemical_relationship","label":"ZIP14 helped kidney-derived cells take up manganese from their lumen-facing side.","description":"ZIP14-specific siRNA reduced apical manganese uptake in polarized mouse proximal-tubule cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9e0e70ec-aa5b-584d-a6ea-28c400cb3100","slug":"mouse-slc39a14","display_name":"Mouse ZIP14 (Slc39a14)","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ef134340-d6ae-5c8f-8b76-303bcc66760a","slug":"cellular-manganese-uptake","display_name":"Cellular manganese uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Transporter-specific siRNA in polarized mouse kidney proximal-tubule culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"ZIP14 siRNA versus controls during apical manganese exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell culture supports an uptake contribution; it does not quantify in vivo urinary reabsorption or rank transporter contributions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Manganese research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"manganese","display_name":"Manganese","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"ZIP14 helped kidney-derived cells take up manganese from their lumen-facing side.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mn-trans-22534978] Roles of ZIP8, ZIP14, and DMT1 in transport of cadmium and manganese in mouse kidney proximal tubule cells. (2012). https://pubmed.ncbi.nlm.nih.gov/22534978/ DOI: 10.1039/c2mt20024d","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Proximal-tubule epithelial cells, apical membrane","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"34f1ed9e-c61f-5335-b464-88191a85425d","evidence_kind":"source_excerpt","locator":"Lines 331-342","start_line":331,"end_line":342,"excerpt":"### mn-trans-renal-zip14\nZIP14-specific siRNA reduced apical manganese uptake in polarized mouse proximal-tubule cells.\nCondition category: machinery_impairment\nnutrient_topic: Manganese research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: ZIP14 helped kidney-derived cells take up manganese from their lumen-facing side.\norganism: Mus musculus\ntissue_or_cell_type: Proximal-tubule epithelial cells, apical membrane\nexperimental_model: Transporter-specific siRNA in polarized mouse kidney proximal-tubule culture\nlimitations: Cell culture supports an uptake contribution; it does not quantify in vivo urinary reabsorption or rank transporter contributions.\nexposure: ZIP14 siRNA versus controls during apical manganese exposure.\ncross_nutrient: false\n[mn-trans-22534978] Roles of ZIP8, ZIP14, and DMT1 in transport of cadmium and manganese in mouse kidney proximal tubule cells. 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