{"id":"313b5444-e869-596e-adf5-7968bffddcb2","stable_key":"e13b03e1-a614-543a-ac1b-97df18cfe30d:iron-nfs1-plp","predicate":"uses","statement":"The NFS1-containing desulfurase complex structure resolved a solvent-exposed PLP cofactor and an unusual substrate-channel architecture.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"cde25631-bef6-5ac2-af1c-cce4b9f75987","mechanism_event_label":"The sulfur-supplying machinery for iron-sulfur clusters also uses a vitamin B6-derived cofactor.","subject":{"id":"9838e184-216a-5a26-a8c5-1777b9dd2d90","slug":"nfs1","display_name":"Human cysteine desulfurase / NFS1","entity_type_key":"protein"},"object":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"cde25631-bef6-5ac2-af1c-cce4b9f75987","stable_key":"e13b03e1-a614-543a-ac1b-97df18cfe30d:iron-nfs1-plp-event","event_type":"biochemical_relationship","label":"The sulfur-supplying machinery for iron-sulfur clusters also uses a vitamin B6-derived cofactor.","description":"The NFS1-containing desulfurase complex structure resolved a solvent-exposed PLP cofactor and an unusual substrate-channel architecture.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c78d3c3c-59b9-5192-b7ec-498124752c92","slug":"lyrm4","display_name":"Human ISD11 / LYRM4","entity_type_key":"protein"},"role":"partner","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"34d475be-4832-5751-9aef-9c92a38254e7","slug":"iron-sulfur-cluster-assembly","display_name":"Iron-sulfur cluster biosynthesis and assembly","entity_type_key":"cellular_process"},"role":"downstream_process","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9838e184-216a-5a26-a8c5-1777b9dd2d90","slug":"nfs1","display_name":"Human cysteine desulfurase / NFS1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/iron-research/28634302.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"82faf07d78311d063c1d990ebb922062650054be372fb324fa67020790c9fa04\", \"start_char\": 0, \"end_char\": 1850, \"text_sha256\": \"82faf07d78311d063c1d990ebb922062650054be372fb324fa67020790c9fa04\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Crystallography, electron microscopy, kinetics and cell studies","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"SDA-complex structural analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Hybrid structural system: bacterial ACP must not be silently labeled human NDUFAB1. Direct dietary B6/B5 effects were not tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human NFS1/ISD11 with bacterial ACP in the recombinant structural complex","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The sulfur-supplying machinery for iron-sulfur clusters also uses a vitamin B6-derived cofactor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iron-p28634302] Structure of human Fe-S assembly subcomplex reveals unexpected cysteine desulfurase architecture and acyl-ACP-ISD11 interactions. (2017). https://pubmed.ncbi.nlm.nih.gov/28634302/ DOI: 10.1073/pnas.1702849114","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Mitochondrial Fe-S assembly machinery","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6bd44955-433f-5521-9918-67b8d52a0d6f","evidence_kind":"source_excerpt","locator":"Lines 1148-1159","start_line":1148,"end_line":1159,"excerpt":"### iron-nfs1-plp\nThe NFS1-containing desulfurase complex structure resolved a solvent-exposed PLP cofactor and an unusual substrate-channel architecture.\nCondition category: normal\nnutrient_topic: Iron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The sulfur-supplying machinery for iron-sulfur clusters also uses a vitamin B6-derived cofactor.\norganism: Human NFS1/ISD11 with bacterial ACP in the recombinant structural complex\ntissue_or_cell_type: Mitochondrial Fe-S assembly machinery\nexperimental_model: Crystallography, electron microscopy, kinetics and cell studies\nlimitations: Hybrid structural system: bacterial ACP must not be silently labeled human NDUFAB1. Direct dietary B6/B5 effects were not tested.\nexposure: SDA-complex structural analysis\nevidence_span: {\"source_cache\": \"artifacts/iron-research/28634302.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"82faf07d78311d063c1d990ebb922062650054be372fb324fa67020790c9fa04\", \"start_char\": 0, \"end_char\": 1850, \"text_sha256\": \"82faf07d78311d063c1d990ebb922062650054be372fb324fa67020790c9fa04\"}\n[iron-p28634302] Structure of human Fe-S assembly subcomplex reveals unexpected cysteine desulfurase architecture and acyl-ACP-ISD11 interactions. (2017). https://pubmed.ncbi.nlm.nih.gov/28634302/ DOI: 10.1073/pnas.1702849114","model_system":"Crystallography, electron microscopy, kinetics and cell studies","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [iron-p28634302] Structure of human Fe-S assembly subcomplex reveals unexpected cysteine desulfurase architecture and acyl-ACP-ISD11 interactions. (2017). https://pubmed.ncbi.nlm.nih.gov/28634302/ DOI: 10.1073/pnas.1702849114","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1560864c-91d1-58dc-90c4-49bf61aba3de","stable_key":"import-e13b03e1-a614-543a-ac1b-97df18cfe30d","title":"Iron: absorption, trafficking, iron-dependent enzymes and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f9dab767a21c9bbfef3227707fe3c82953536bb1335599dd4ef93b5ea2dbf03f","revision_id":"77700d7f-2902-54b9-adae-8cbc77b24682","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}