{"id":"30f3d727-fe73-5f73-84a5-8229e796b06a","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-ampk-reactivation","predicate":"supports","statement":"AMPK loss impaired mTORC1 reactivation during prolonged amino-acid deprivation and increased apoptosis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d9132d1f-983f-5e19-8e06-60e8e1ab8aaf","mechanism_event_label":"The same sensor can support recovery during persistent stress.","subject":{"id":"8128eec0-61cc-51ef-8d4e-5ec9678e8dfa","slug":"ampk-complexes","display_name":"AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"object":{"id":"affc7d33-29a3-515c-b62b-f8b4b897c543","slug":"mtorc1-reactivation","display_name":"mTORC1 reactivation after prolonged amino-acid withdrawal","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d9132d1f-983f-5e19-8e06-60e8e1ab8aaf","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-ampk-reactivation-event","event_type":"observed_relationship","label":"The same sensor can support recovery during persistent stress.","description":"AMPK loss impaired mTORC1 reactivation during prolonged amino-acid deprivation and increased apoptosis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8128eec0-61cc-51ef-8d4e-5ec9678e8dfa","slug":"ampk-complexes","display_name":"AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"affc7d33-29a3-515c-b62b-f8b4b897c543","slug":"mtorc1-reactivation","display_name":"mTORC1 reactivation after prolonged amino-acid withdrawal","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9f025af3-f039-517f-908b-4af6fc65415e","slug":"mtorc1","display_name":"Mechanistic target of rapamycin complex 1","entity_type_key":"protein_complex"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2bf4b948-c657-531a-967c-9b180f14bff1","slug":"stress-induced-apoptosis","display_name":"Apoptosis during prolonged nutrient stress","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"78a748c5-cbd6-5b85-89e9-d60e9f59bebb","slug":"experimental-amino-acid-deprivation","display_name":"Experimental amino-acid deprivation","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cultured nutrient-stressed cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell survival and autophagy readouts must be distinguished.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fasting physiological-state collection; human protocols, cellular deprivation and refeeding are distinguished.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fasting","display_name":"Fasting / abstention from energy intake","entity_type_key":"cellular_process"}},{"dimension":"plain_language","value_text":"The same sensor can support recovery during persistent stress.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Unexpected roles for AMPK in the suppression of autophagy and the reactivation of MTORC1 signaling during prolonged amino acid deprivation. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38744665/ · DOI 10.1080/15548627.2024.2355074","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"85643a22-9a67-50b7-b897-5f14ceb2b428","evidence_kind":"source_excerpt","locator":"Lines 320-326","start_line":320,"end_line":326,"excerpt":"## fast-ampk-reactivation\nThe same sensor can support recovery during persistent stress.\nAMPK loss impaired mTORC1 reactivation during prolonged amino-acid deprivation and increased apoptosis.\nModel: Cultured nutrient-stressed cells.\nLimitations: Cell survival and autophagy readouts must be distinguished.\nEvidence access: Primary abstract\nUnexpected roles for AMPK in the suppression of autophagy and the reactivation of MTORC1 signaling during prolonged amino acid deprivation. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38744665/ · DOI 10.1080/15548627.2024.2355074","model_system":"Cultured nutrient-stressed cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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