{"id":"30dccaa4-27fb-544f-97cb-547c083b84d3","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-muscle-ampk","predicate":"did_not_change","statement":"Measured muscle AMPK activity was unchanged.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"54dcde7e-13f2-5ef8-b786-9083db4757e6","mechanism_event_label":"A popular fasting signal did not rise in this tissue and protocol.","subject":{"id":"2d5df4b9-44d7-56a5-8ecf-7c08c0ae9f4f","slug":"fasting","display_name":"Fasting / abstention from energy intake","entity_type_key":"cellular_process"},"object":{"id":"59b7c30f-59d4-5eab-b5b0-1d0cc330d200","slug":"skeletal-muscle-ampk-activity","display_name":"Skeletal-muscle AMPK activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"54dcde7e-13f2-5ef8-b786-9083db4757e6","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-muscle-ampk-event","event_type":"observed_relationship","label":"A popular fasting signal did not rise in this tissue and protocol.","description":"Measured muscle AMPK activity was unchanged.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2d5df4b9-44d7-56a5-8ecf-7c08c0ae9f4f","slug":"fasting","display_name":"Fasting / abstention from energy intake","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"59b7c30f-59d4-5eab-b5b0-1d0cc330d200","slug":"skeletal-muscle-ampk-activity","display_name":"Skeletal-muscle AMPK activity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"95bc303f-ad04-59ea-8e1e-32f9e8b72c56","slug":"human-ampk-complexes","display_name":"Human AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Thirteen adults; seven-day fast, muscle biopsies and exercise testing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not proof that AMPK is unchanged in every tissue or at every time point.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fasting physiological-state collection; human protocols, cellular deprivation and refeeding are distinguished.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fasting","display_name":"Fasting / abstention from energy intake","entity_type_key":"cellular_process"}},{"dimension":"plain_language","value_text":"A popular fasting signal did not rise in this tissue and protocol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Effects of seven days' fasting on physical performance and metabolic adaptation during exercise in humans. · 2025 · https://pubmed.ncbi.nlm.nih.gov/39747857/ · DOI 10.1038/s41467-024-55418-0","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"80622740-f183-55ba-b4a6-9fe27eac653d","evidence_kind":"source_excerpt","locator":"Lines 184-190","start_line":184,"end_line":190,"excerpt":"## fast-muscle-ampk\nA popular fasting signal did not rise in this tissue and protocol.\nMeasured muscle AMPK activity was unchanged.\nModel: Thirteen adults; seven-day fast, muscle biopsies and exercise testing.\nLimitations: Not proof that AMPK is unchanged in every tissue or at every time point.\nEvidence access: Primary abstract\nEffects of seven days' fasting on physical performance and metabolic adaptation during exercise in humans. · 2025 · https://pubmed.ncbi.nlm.nih.gov/39747857/ · DOI 10.1038/s41467-024-55418-0","model_system":"Thirteen adults; seven-day fast, muscle biopsies and exercise testing.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"921d1eb5-2c88-52b9-9898-60b2b5bf6029","stable_key":"import-c9aa15a1-8ba2-5913-b2dd-372af304bf87","title":"Fasting: fuel switching, nutrient sensing, ketone signaling, nutrient dependencies and refeeding (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"991b7fd94be84c0f1e622e0672eda03d600b388dfd7825e219aa1678d1c7983a","revision_id":"ce85d3f4-4eaf-5aee-bddb-0f0ce7fe2211","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}