{"id":"30d92738-e565-5b67-887a-eef3b1adb452","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-vrk1-baf","predicate":"reduces_vrk1_phosphorylation","statement":"Luteolin reduced VRK1-mediated BAF phosphorylation; 10 micromolar treatment altered nuclear morphology in HeLa cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b576cdc7-2354-54ca-ae6a-e2919d511b24","mechanism_event_label":"Kinase inhibition changed a nuclear-envelope regulator.","subject":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"object":{"id":"a6ba357c-7394-5e70-bc77-48a80c6c7780","slug":"banf1","display_name":"Human barrier-to-autointegration factor / BANF1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"b576cdc7-2354-54ca-ae6a-e2919d511b24","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-vrk1-baf-event","event_type":"observed_relationship","label":"Kinase inhibition changed a nuclear-envelope regulator.","description":"Luteolin reduced VRK1-mediated BAF phosphorylation; 10 micromolar treatment altered nuclear morphology in HeLa cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a6ba357c-7394-5e70-bc77-48a80c6c7780","slug":"banf1","display_name":"Human barrier-to-autointegration factor / BANF1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2c01de74-8b26-5489-824c-6b08d9958cbf","slug":"vrk1","display_name":"Human vaccinia-related kinase 1 / VRK1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant kinase and human HeLa-cell experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not a selective VRK1 inhibitor or a demonstrated cancer therapy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Kinase inhibition changed a nuclear-envelope regulator.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Luteolin suppresses cancer cell proliferation by targeting vaccinia-related kinase 1. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25310002/ · DOI 10.1371/journal.pone.0109655","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d90eeb36-3e35-5373-9179-0fa16b30e93b","evidence_kind":"source_excerpt","locator":"Lines 356-362","start_line":356,"end_line":362,"excerpt":"## luteolin-vrk1-baf\nKinase inhibition changed a nuclear-envelope regulator.\nLuteolin reduced VRK1-mediated BAF phosphorylation; 10 micromolar treatment altered nuclear morphology in HeLa cells.\nModel: Recombinant kinase and human HeLa-cell experiments.\nLimitations: Not a selective VRK1 inhibitor or a demonstrated cancer therapy.\nEvidence access: Primary full text\nLuteolin suppresses cancer cell proliferation by targeting vaccinia-related kinase 1. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25310002/ · DOI 10.1371/journal.pone.0109655","model_system":"Recombinant kinase and human HeLa-cell experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"fd6ea0b1cef8d1c9965270494bfa815de14f679044f0b5d5d7bbf4904630ad76","revision_id":"c9a3dd80-c7e6-5fb4-bc91-8db265e7c8de","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}