{"id":"30c2f842-63f3-58f4-912f-81e79f6ddfd0","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:lox-peptidyl-lysine-oxidation","predicate":"oxidatively_deaminates","statement":"Lysyl oxidase converts suitable peptidyl lysines to allysine, producing ammonia and hydrogen peroxide.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"cd66a45b-7701-5724-9aab-807fa4e64da6","mechanism_event_label":"An enzyme creates reactive attachment sites used in matrix cross-linking.","subject":{"id":"815f6321-8845-5ab5-bbd2-90872ac2d032","slug":"lysyl-oxidase-family","display_name":"Lysyl oxidase enzyme family","entity_type_key":"protein_family"},"object":{"id":"525d733c-8646-56cf-ba01-a38b28f77aa6","slug":"ecm-protein-lysine","display_name":"Collagen/elastin peptidyl lysine substrates","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"cd66a45b-7701-5724-9aab-807fa4e64da6","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:lox-peptidyl-lysine-oxidation-event","event_type":"biochemical_relationship","label":"An enzyme creates reactive attachment sites used in matrix cross-linking.","description":"Lysyl oxidase converts suitable peptidyl lysines to allysine, producing ammonia and hydrogen peroxide.","status":"provisional","compartment":{"slug":"extracellular-space","display_name":"Extracellular space"},"participants":[{"entity":{"id":"815f6321-8845-5ab5-bbd2-90872ac2d032","slug":"lysyl-oxidase-family","display_name":"Lysyl oxidase enzyme family","entity_type_key":"protein_family"},"role":"input","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"525d733c-8646-56cf-ba01-a38b28f77aa6","slug":"ecm-protein-lysine","display_name":"Collagen/elastin peptidyl lysine substrates","entity_type_key":"protein_state"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bdd7dfe3-b5f2-55e0-a28d-c67a33c47fb3","slug":"loxl2","display_name":"LOXL2","entity_type_key":"protein"},"role":"experimentally_tested_family_member","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6885b858-dcfa-5dae-adf0-c24c2d19120a","slug":"ecm-protein-allysine","display_name":"Collagen/elastin peptidyl allysine products","entity_type_key":"protein_state"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"899c7ab0-6f81-5b38-a6bd-fbb33d66ac8d","slug":"oxygen","display_name":"Molecular oxygen","entity_type_key":"small_molecule"},"role":"cosubstrate","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"d31225b7-8e94-5857-9ea1-67ac794b1f15","slug":"water","display_name":"H2O","entity_type_key":"small_molecule"},"role":"cosubstrate","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"1374ec24-1a5b-552b-982f-51f9082f4187","slug":"ammonia","display_name":"Ammonia","entity_type_key":"small_molecule"},"role":"coproduct","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"coproduct","stoichiometry":null,"state_label":"","sequence_order":7,"notes":""},{"entity":{"id":"2a870bb5-05a0-5e51-a70c-a9a843a8b571","slug":"copper-ii","display_name":"Copper(II) ion","entity_type_key":"ion"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":8,"notes":""},{"entity":{"id":"17e1311d-64f9-5f5a-9281-f188a9afcf9f","slug":"ltq-cofactor","display_name":"Protein-bound lysine tyrosylquinone","entity_type_key":"protein_state"},"role":"protein_derived_cofactor","stoichiometry":null,"state_label":"","sequence_order":9,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"LOXL2 assay development and total-family activity detection in cultured cells and tissue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Substrate sites and enzyme-family members differ; peroxide production alone does not establish systemic oxidative injury.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Mammalian cells/tissues and recombinant LOXL2; see study methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"An enzyme creates reactive attachment sites used in matrix cross-linking.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lox-assay-2021] An in situ activity assay for lysyl oxidases (2021). https://pubmed.ncbi.nlm.nih.gov/34226627/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Not specified as a whole tissue; see experimental model.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"88951ca2-09d2-5aa4-bc0f-f2b773b9999e","evidence_kind":"source_excerpt","locator":"Lines 467-475","start_line":467,"end_line":475,"excerpt":"### lox-peptidyl-lysine-oxidation\nLysyl oxidase converts suitable peptidyl lysines to allysine, producing ammonia and hydrogen peroxide.\nPlain language: An enzyme creates reactive attachment sites used in matrix cross-linking.\nCondition category: normal\norganism: Mammalian cells/tissues and recombinant LOXL2; see study methods\ntissue_or_cell_type: Not specified as a whole tissue; see experimental model.\nexperimental_model: LOXL2 assay development and total-family activity detection in cultured cells and tissue.\nlimitations: Substrate sites and enzyme-family members differ; peroxide production alone does not establish systemic oxidative injury.\n[lox-assay-2021] An in situ activity assay for lysyl oxidases (2021). https://pubmed.ncbi.nlm.nih.gov/34226627/","model_system":"LOXL2 assay development and total-family activity detection in cultured cells and tissue.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [lox-assay-2021] An in situ activity assay for lysyl oxidases (2021). https://pubmed.ncbi.nlm.nih.gov/34226627/","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}