{"id":"30874608-f289-58e0-91a5-ed02f312d6ad","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:va-repro-human-stra8-induction","predicate":"increases_expression","statement":"RA increased STRA8 RNA 2.2-fold in cultured human fetal testis cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"c5ec4181-bdd4-56a0-a312-49a34a166f2a","mechanism_event_label":"Human fetal testis cells can respond to RA by increasing STRA8.","subject":{"id":"05487b29-8c59-5af8-83cf-cf9c79c5ecb8","slug":"all-trans-retinoic-acid","display_name":"All-trans-retinoic acid","entity_type_key":"small_molecule"},"object":{"id":"71ef3a9a-f42c-59ea-a2b2-28d02e4c305b","slug":"human-stra8-expression","display_name":"Human STRA8 expression","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c5ec4181-bdd4-56a0-a312-49a34a166f2a","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:va-repro-human-stra8-induction-event","event_type":"biochemical_relationship","label":"Human fetal testis cells can respond to RA by increasing STRA8.","description":"RA increased STRA8 RNA 2.2-fold in cultured human fetal testis cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8394f01d-62c5-5350-9e5e-60d7b0acc441","slug":"stra8","display_name":"Human STRA8","entity_type_key":"protein"},"role":"encoded_regulator","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"05487b29-8c59-5af8-83cf-cf9c79c5ecb8","slug":"all-trans-retinoic-acid","display_name":"All-trans-retinoic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"71ef3a9a-f42c-59ea-a2b2-28d02e4c305b","slug":"human-stra8-expression","display_name":"Human STRA8 expression","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Disaggregated fetal testis culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"14-15 weeks gestation; 1 micromolar RA for 24 h; n=5 for STRA8.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This transcript response does not establish entry into meiosis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Human fetal testis cells can respond to RA by increasing STRA8.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[human2011] Retinoic Acid signalling and the control of meiotic entry in the human fetal gonad. (2011). https://pubmed.ncbi.nlm.nih.gov/21674038/ DOI: 10.1371/journal.pone.0020249","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"human fetal testis","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"99369979-f672-5ec5-81f2-b76bc8b5fde0","evidence_kind":"source_excerpt","locator":"Lines 330-341","start_line":330,"end_line":341,"excerpt":"### va-repro-human-stra8-induction\nRA increased STRA8 RNA 2.2-fold in cultured human fetal testis cells.\nCondition category: normal\nnutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Human fetal testis cells can respond to RA by increasing STRA8.\norganism: Homo sapiens\ntissue_or_cell_type: human fetal testis\nexperimental_model: Disaggregated fetal testis culture\nlimitations: This transcript response does not establish entry into meiosis.\nexposure: 14-15 weeks gestation; 1 micromolar RA for 24 h; n=5 for STRA8.\ncross_nutrient: false\n[human2011] Retinoic Acid signalling and the control of meiotic entry in the human fetal gonad. (2011). https://pubmed.ncbi.nlm.nih.gov/21674038/ DOI: 10.1371/journal.pone.0020249","model_system":"Disaggregated fetal testis culture","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [human2011] Retinoic Acid signalling and the control of meiotic entry in the human fetal gonad. (2011). https://pubmed.ncbi.nlm.nih.gov/21674038/ DOI: 10.1371/journal.pone.0020249","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"83131a07-353f-5301-9ce6-50754c194952","stable_key":"import-3c852f36-7951-5fd9-9013-d771a8d16d8a","title":"Vitamin A expansion: reproduction, light-driven vision and shared mechanisms (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"559cb4dc3f59519ecb0bc455dbcc73346fbf8e0c51e133f455781f51e4ed07ab","revision_id":"225fe4da-2d21-5c19-a92c-80a5f3cac8cb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}