{"id":"306d72c2-33c4-5fef-8818-fe43e5eeb008","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:cyanidin-iron-chelation-assay","predicate":"reported_relationship","statement":"Purified cyanidin bound iron in the reported cell-free assays.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"6741e82f-8625-5de5-a0de-8f737a8dbf91","mechanism_event_label":"Purified cyanidin bound iron in the reported cell-free assays.","subject":{"id":"96034147-6771-585d-83da-c450a4313d80","slug":"cyanidin","display_name":"Cyanidin","entity_type_key":"small_molecule"},"object":{"id":"cbdbb79e-5dcd-5b53-a08e-f11e5333f872","slug":"iron-chelation-assay","display_name":"Iron complexation in cell-free chemical assays","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6741e82f-8625-5de5-a0de-8f737a8dbf91","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:cyanidin-iron-chelation-assay-event","event_type":"biochemical_relationship","label":"Purified cyanidin bound iron in the reported cell-free assays.","description":"**Iron binding and metal reduction are different reactions.** Purified cyanidin, C3G and standardized elderberry extracts chelated iron and reduced iron and copper in cell-free assays; copper-chelating activity was relatively low. Extract behavior varied and was not completely explained by pigment concentration. Binding a metal, changing its oxidation state and reducing intestinal absorption are three different endpoints. This does not establish removal of intracellular metals or protection from ferroptosis in people. [Mladěnka et al., 2016](https://doi.org/10.1016/j.phytol.2016.10.025).","status":"provisional","compartment":null,"participants":[{"entity":{"id":"96034147-6771-585d-83da-c450a4313d80","slug":"cyanidin","display_name":"Cyanidin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"cbdbb79e-5dcd-5b53-a08e-f11e5333f872","slug":"iron-chelation-assay","display_name":"Iron complexation in cell-free chemical assays","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""},{"entity":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"role":"assay metal, oxidation state and speciation depend on protocol","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary article abstract read in indexed publisher/author-hosted result; publisher full text unavailable. Exact pH series and extract/metal ratios not extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-free spectrophotometric metal assays; multiple pH and compound/metal ratios; exact series not extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Binding or reduction, not human absorption, intracellular stripping or ferroptosis prevention. 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Extract behavior varied and was not completely explained by pigment concentration. Binding a metal, changing its oxidation state and reducing intestinal absorption are three different endpoints. This does not establish removal of intracellular metals or protection from ferroptosis in people. [Mladěnka et al., 2016](https://doi.org/10.1016/j.phytol.2016.10.025).","model_system":"Cell-free spectrophotometric metal assays; multiple pH and compound/metal ratios; exact series not extracted.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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