{"id":"305e8fa9-34cb-539e-bd73-8f34fb2a0c95","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ve-transport-rat-ttp-affinity-gamma-tocopherol","predicate":"binds_with_lower_relative_affinity","statement":"In a rat alpha-TTP membrane-transfer competition assay, gamma-tocopherol had relative affinity 8.9% of RRR-alpha-tocopherol (100%).","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5e327780-6556-5e6d-9c6b-5f5301619c09","mechanism_event_label":"Rat alpha-TTP distinguished this form from RRR-alpha-tocopherol.","subject":{"id":"f6f0738e-b010-543f-9dfc-e4450b528fee","slug":"rat-ttpa","display_name":"Rat alpha-tocopherol transfer protein","entity_type_key":"protein"},"object":{"id":"2de3a4d0-56b1-57f9-9e9d-934ba52a1753","slug":"gamma-tocopherol","display_name":"Gamma-tocopherol","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"5e327780-6556-5e6d-9c6b-5f5301619c09","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ve-transport-rat-ttp-affinity-gamma-tocopherol-event","event_type":"biochemical_relationship","label":"Rat alpha-TTP distinguished this form from RRR-alpha-tocopherol.","description":"In a rat alpha-TTP membrane-transfer competition assay, gamma-tocopherol had relative affinity 8.9% of RRR-alpha-tocopherol (100%).","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f6f0738e-b010-543f-9dfc-e4450b528fee","slug":"rat-ttpa","display_name":"Rat alpha-tocopherol transfer protein","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2de3a4d0-56b1-57f9-9e9d-934ba52a1753","slug":"gamma-tocopherol","display_name":"Gamma-tocopherol","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae6048a7-d112-5d3a-8e7c-ad5a152642f9","slug":"rrr-alpha-tocopherol","display_name":"RRR-alpha-tocopherol","entity_type_key":"small_molecule"},"role":"reference_ligand","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified rat liver protein and membrane-transfer competition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"37 °C, 30 min; Table 1 competition-derived affinity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Relative transfer competition, not a direct Kd or human clinical efficacy ratio.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Rat alpha-TTP distinguished this form from RRR-alpha-tocopherol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[hosomi1997] Affinity for alpha-tocopherol transfer protein as a determinant of the biological activities of vitamin E analogs. (1997). https://pubmed.ncbi.nlm.nih.gov/9199513/ DOI: 10.1016/s0014-5793(97)00499-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver protein/liposomes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f8e601ff-8a90-565d-96f9-7eab69091162","evidence_kind":"source_excerpt","locator":"Lines 155-166","start_line":155,"end_line":166,"excerpt":"### ve-transport-rat-ttp-affinity-gamma-tocopherol\nIn a rat alpha-TTP membrane-transfer competition assay, gamma-tocopherol had relative affinity 8.9% of RRR-alpha-tocopherol (100%).\nCondition category: normal\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Rat alpha-TTP distinguished this form from RRR-alpha-tocopherol.\norganism: Rattus norvegicus\ntissue_or_cell_type: Liver protein/liposomes\nexperimental_model: Purified rat liver protein and membrane-transfer competition\nlimitations: Relative transfer competition, not a direct Kd or human clinical efficacy ratio.\nexposure: 37 °C, 30 min; Table 1 competition-derived affinity.\ncross_nutrient: false\n[hosomi1997] Affinity for alpha-tocopherol transfer protein as a determinant of the biological activities of vitamin E analogs. 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