{"id":"302b9851-2bff-516b-93ab-70847de447e6","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-copper-dna","predicate":"promotes","statement":"Resveratrol/copper treatment produced plasmid sequence mutations and reduced transformation efficiency in a cell-free DNA/bacterial readout experiment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3c26298a-cc95-56d9-8589-737ed865e22c","mechanism_event_label":"Copper can change an antioxidant into a pro-oxidant in an assay.","subject":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"object":{"id":"be962e0e-35f9-55bd-917d-21397fe0f6c7","slug":"resveratrol-copper-plasmid-damage","display_name":"Plasmid DNA injury during resveratrol/copper exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3c26298a-cc95-56d9-8589-737ed865e22c","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-copper-dna-event","event_type":"observed_relationship","label":"Copper can change an antioxidant into a pro-oxidant in an assay.","description":"Resveratrol/copper treatment produced plasmid sequence mutations and reduced transformation efficiency in a cell-free DNA/bacterial readout experiment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"be962e0e-35f9-55bd-917d-21397fe0f6c7","slug":"resveratrol-copper-plasmid-damage","display_name":"Plasmid DNA injury during resveratrol/copper exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2a870bb5-05a0-5e51-a70c-a9a843a8b571","slug":"copper-ii","display_name":"Copper(II) ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Plasmid treatment followed by E. coli transformation/sequencing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not demonstrated human mutagenicity from dietary resveratrol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Resveratrol collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Copper can change an antioxidant into a pro-oxidant in an assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Prooxidant activity of resveratrol in the presence of copper ions: mutagenicity in plasmid DNA. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15913925/ · DOI 10.1016/j.toxlet.2005.04.001","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"11d57120-927a-57e6-906d-fa4258dd107b","evidence_kind":"source_excerpt","locator":"Lines 366-372","start_line":366,"end_line":372,"excerpt":"## resveratrol-copper-dna\nCopper can change an antioxidant into a pro-oxidant in an assay.\nResveratrol/copper treatment produced plasmid sequence mutations and reduced transformation efficiency in a cell-free DNA/bacterial readout experiment.\nModel: Plasmid treatment followed by E. coli transformation/sequencing.\nLimitations: Not demonstrated human mutagenicity from dietary resveratrol.\nEvidence access: Primary abstract\nProoxidant activity of resveratrol in the presence of copper ions: mutagenicity in plasmid DNA. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15913925/ · DOI 10.1016/j.toxlet.2005.04.001","model_system":"Plasmid treatment followed by E. coli transformation/sequencing.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2844eda9-a122-5357-ae47-cd58227c5994","stable_key":"import-52871722-4aa6-5453-a902-3e76356db327","title":"Resveratrol: metabolites, target selectivity and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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