{"id":"2ff12e3b-3d53-5414-9dc1-e47cef7aa2cd","stable_key":"708c252b-0aef-5cec-95bf-99d19578214c:ml349-stat3-palmit","predicate":"has_contextual_experimental_result","statement":"ML349 increased STAT3 palmitoylation in the reported reconstituted HEK293T experiment.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"0cfb15eb-d3ba-58f3-af23-41c739620952","mechanism_event_label":"ml349-stat3-palmit","subject":{"id":"a160f6fb-092c-5df1-927c-4354e3af03f2","slug":"ml349","display_name":"ML349, experimental APT2 inhibitor","entity_type_key":"small_molecule"},"object":{"id":"2bb7e799-f77a-5a72-b8d6-3ec458cc11aa","slug":"human-stat3-palmitoylation","display_name":"STAT3 palmitoylation in human cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0cfb15eb-d3ba-58f3-af23-41c739620952","stable_key":"708c252b-0aef-5cec-95bf-99d19578214c:ml349-stat3-palmit","event_type":"experimental_result","label":"ml349-stat3-palmit","description":"ML349 increased STAT3 palmitoylation in the reported reconstituted HEK293T experiment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a160f6fb-092c-5df1-927c-4354e3af03f2","slug":"ml349","display_name":"ML349, experimental APT2 inhibitor","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2bb7e799-f77a-5a72-b8d6-3ec458cc11aa","slug":"human-stat3-palmitoylation","display_name":"STAT3 palmitoylation in human cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f2ffef5b-5457-50a8-b787-b8f24430f610","slug":"lypla2","display_name":"LYPLA2","entity_type_key":"protein"},"role":"pharmacological target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"dc129d3d-aaa0-5511-9a60-3e3880b0eb0f","slug":"stat3","display_name":"STAT3","entity_type_key":"protein"},"role":"measured protein","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human HEK293T; DHHC7 knockout reconstituted with HA-DHHC7 and Flag-STAT3; DHHC construct species is not established as human for this figure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"20 micromolar ML349 for 36 hours before Alk14 labeling; labeling methods specify 50 micromolar Alk14 for 5 hours.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Reported cellular result, not a GPX4 time-course experiment. Overexpressed DHHC constructs in much of the study are mouse proteins; human constructs are explicitly identified only where confirmed. 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Overexpressed DHHC constructs in much of the study are mouse proteins; human constructs are explicitly identified only where confirmed. 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