{"id":"2fd1c14a-2e13-5ae8-9d31-95f3202ebc0f","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-cars-camkk2-bridge","predicate":"connects_ampk_to","statement":"Under cysteine-deficient conditions, CARS promoted AMPK association with CaMKK2 and CaMKK2-dependent AMPK phosphorylation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"074dfb9c-fd44-5555-831c-4460d984fc78","mechanism_event_label":"The sensing protein helped bring a kinase and its target together.","subject":{"id":"a520643d-30d3-5db6-b967-fcc359e4b6b2","slug":"cars1","display_name":"Human cytosolic cysteinyl-tRNA synthetase / CARS1","entity_type_key":"protein"},"object":{"id":"002c9475-0df2-52d4-bbbb-5649661b2340","slug":"camkk2","display_name":"Human calcium/calmodulin-dependent protein kinase kinase 2 / CAMKK2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"074dfb9c-fd44-5555-831c-4460d984fc78","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-cars-camkk2-bridge-event","event_type":"observed_relationship","label":"The sensing protein helped bring a kinase and its target together.","description":"Under cysteine-deficient conditions, CARS promoted AMPK association with CaMKK2 and CaMKK2-dependent AMPK phosphorylation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a520643d-30d3-5db6-b967-fcc359e4b6b2","slug":"cars1","display_name":"Human cytosolic cysteinyl-tRNA synthetase / CARS1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"002c9475-0df2-52d4-bbbb-5649661b2340","slug":"camkk2","display_name":"Human calcium/calmodulin-dependent protein kinase kinase 2 / CAMKK2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bcb2ae22-0b0b-56d0-8847-6540eb217080","slug":"prkag2","display_name":"Human AMPK gamma-2 / PRKAG2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"95bc303f-ad04-59ea-8e1e-32f9e8b72c56","slug":"human-ampk-complexes","display_name":"Human AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; 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primary references, access levels and experimental limitations individually identified. 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