{"id":"2fb5a8fe-4333-5508-97dd-a638c0ee1f00","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-ctns-proton-switch","predicate":"regulates_conformation","statement":"D346N substitution abolished the pH-dependent conformational switch; the authors proposed D346 as a key protonation site controlling human cystinosin transitions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4b3e20c3-b330-5db8-b19b-4156c8bdad75","mechanism_event_label":"The transporter couples substrate movement to an acidic compartment.","subject":{"id":"0d4adb17-eb94-510a-b5dc-50e44ba6f088","slug":"human-ctns-d346-protonation","display_name":"Proposed human CTNS D346 protonation switch","entity_type_key":"cellular_process"},"object":{"id":"80987fa1-4f0c-5456-a6e2-d7f7d2d493b3","slug":"ctns","display_name":"Human lysosomal cystine transporter / CTNS","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"4b3e20c3-b330-5db8-b19b-4156c8bdad75","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-ctns-proton-switch-event","event_type":"observed_relationship","label":"The transporter couples substrate movement to an acidic compartment.","description":"D346N substitution abolished the pH-dependent conformational switch; 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