{"id":"2fb399d7-c0a5-5fb4-87a5-3c07cb39028b","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vdm-calcitriol-mouse-osteocalcin-culture-scope","predicate":"reduces_accumulation","statement":"In primary calvarial osteoblast cultures from the same transgenic mice, calcitriol inhibited endogenous mouse osteocalcin accumulation while increasing human osteocalcin production.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"bf3813f7-8f5a-5a9f-8fd2-a944d4887331","mechanism_event_label":"Mouse and human osteocalcin genes responded differently in the same experimental setting.","subject":{"id":"f5156c24-108f-5746-b114-ed764e79a3f4","slug":"calcitriol","display_name":"Calcitriol","entity_type_key":"small_molecule"},"object":{"id":"b043ce5c-8537-5099-8421-b07ec993dbde","slug":"bglap","display_name":"Osteocalcin / BGLAP","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"bf3813f7-8f5a-5a9f-8fd2-a944d4887331","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vdm-calcitriol-mouse-osteocalcin-culture-scope-event","event_type":"biochemical_relationship","label":"Mouse and human osteocalcin genes responded differently in the same experimental setting.","description":"In primary calvarial osteoblast cultures from the same transgenic mice, calcitriol inhibited endogenous mouse osteocalcin accumulation while increasing human osteocalcin production.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f5156c24-108f-5746-b114-ed764e79a3f4","slug":"calcitriol","display_name":"Calcitriol","entity_type_key":"small_molecule"},"role":"hormonal-regulator","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b043ce5c-8537-5099-8421-b07ec993dbde","slug":"bglap","display_name":"Osteocalcin / BGLAP","entity_type_key":"protein"},"role":"species-resolved-protein","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Vitamin D–vitamin K boundary: gene expression and carboxylation are distinct, and osteocalcin transcription is species-dependent.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_locator","value_text":"Abstract: reported experimental results","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"D3 active metabolite or VDR machinery experiment; not a direct D2-versus-D3 comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary calvarial osteoblasts from human-osteocalcin-transgenic mice; species-specific protein assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Calcitriol exposure of primary cultures; exact culture dose/time not given in the retrieved abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Species and gene-regulatory context explain the difference; this is not an orange scientific conflict and does not predict human fracture outcomes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient","value_text":"Vitamin D2 and D3","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"nutrient_topic","value_text":"Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus with human BGLAP transgene","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Mouse and human osteocalcin genes responded differently in the same experimental setting.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[vdm-zhang1997] Analysis of osteocalcin expression in transgenic mice reveals a species difference in vitamin D regulation of mouse and human osteocalcin genes. (1997). https://pubmed.ncbi.nlm.nih.gov/9333117/ DOI: 10.1359/jbmr.1997.12.10.1570","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Calvarial osteoblast culture","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3917e581-40c7-56fd-bbbf-9e027ae0aa7c","evidence_kind":"source_excerpt","locator":"Lines 873-887","start_line":873,"end_line":887,"excerpt":"### vdm-calcitriol-mouse-osteocalcin-culture-scope\nIn primary calvarial osteoblast cultures from the same transgenic mice, calcitriol inhibited endogenous mouse osteocalcin accumulation while increasing human osteocalcin production.\nCondition category: normal\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Mouse and human osteocalcin genes responded differently in the same experimental setting.\norganism: Mus musculus with human BGLAP transgene\ntissue_or_cell_type: Calvarial osteoblast culture\nexperimental_model: Primary calvarial osteoblasts from human-osteocalcin-transgenic mice; species-specific protein assays\nlimitations: Species and gene-regulatory context explain the difference; this is not an orange scientific conflict and does not predict human fracture outcomes.\nexposure: Calcitriol exposure of primary cultures; exact culture dose/time not given in the retrieved abstract.\ncross_nutrient: Vitamin D–vitamin K boundary: gene expression and carboxylation are distinct, and osteocalcin transcription is species-dependent.\nevidence_locator: Abstract: reported experimental results\nnutrient: Vitamin D2 and D3\nevidence_scope: D3 active metabolite or VDR machinery experiment; not a direct D2-versus-D3 comparison.\n[vdm-zhang1997] Analysis of osteocalcin expression in transgenic mice reveals a species difference in vitamin D regulation of mouse and human osteocalcin genes. (1997). https://pubmed.ncbi.nlm.nih.gov/9333117/ DOI: 10.1359/jbmr.1997.12.10.1570","model_system":"Primary calvarial osteoblasts from human-osteocalcin-transgenic mice; species-specific protein assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [vdm-zhang1997] Analysis of osteocalcin expression in transgenic mice reveals a species difference in vitamin D regulation of mouse and human osteocalcin genes. 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