{"id":"2f8ce0f3-8310-516c-a1d9-fc490a2c1429","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:rat-mthfd2l-oxidation","predicate":"oxidizes","statement":"Rat MTHFD2L catalyzes methylene-THF oxidation with either NAD+ or NADP+.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"06b0e13a-ac81-5426-a827-990c22f39d20","mechanism_event_label":"A second mitochondrial enzyme can use either electron carrier.","subject":{"id":"ab8012eb-70cc-5a65-b8d6-2fb31a7141f5","slug":"rat-mthfd2l","display_name":"Rat mitochondrial MTHFD2L","entity_type_key":"protein"},"object":{"id":"0413b195-325e-5757-a72b-f445d65c17bc","slug":"5-10-methylenetetrahydrofolate","display_name":"5,10-Methylenetetrahydrofolate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"06b0e13a-ac81-5426-a827-990c22f39d20","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:rat-mthfd2l-oxidation-event","event_type":"biochemical_relationship","label":"A second mitochondrial enzyme can use either electron carrier.","description":"Rat MTHFD2L catalyzes methylene-THF oxidation with either NAD+ or NADP+.","status":"provisional","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"acceptor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"be6e4670-953f-5a4e-97da-1e536a54e0e4","slug":"nadp-plus","display_name":"NADP+","entity_type_key":"small_molecule"},"role":"acceptor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7978c99f-fb17-5d91-a9e4-178558bfa69f","slug":"5-10-methenyltetrahydrofolate","display_name":"5,10-Methenyltetrahydrofolate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ab8012eb-70cc-5a65-b8d6-2fb31a7141f5","slug":"rat-mthfd2l","display_name":"Rat mitochondrial MTHFD2L","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"0413b195-325e-5757-a72b-f445d65c17bc","slug":"5-10-methylenetetrahydrofolate","display_name":"5,10-Methylenetetrahydrofolate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Purified recombinant rat protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Assay conditions described in the linked primary study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Human ortholog flux was not measured.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A second mitochondrial enzyme can use either electron carrier.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[shin-2014] Mitochondrial MTHFD2L is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase expressed in both adult and embryonic tissues (2014). https://pubmed.ncbi.nlm.nih.gov/24733394/ DOI: 10.1074/jbc.m114.555573","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell-free","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7bcd2622-ba57-5d1e-bc93-50c8de046a3a","evidence_kind":"source_excerpt","locator":"Lines 838-848","start_line":838,"end_line":848,"excerpt":"### rat-mthfd2l-oxidation\nRat MTHFD2L catalyzes methylene-THF oxidation with either NAD+ or NADP+.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A second mitochondrial enzyme can use either electron carrier.\norganism: Rattus norvegicus\ntissue_or_cell_type: Cell-free\nexperimental_model: Purified recombinant rat protein\nlimitations: Human ortholog flux was not measured.\nexposure: Assay conditions described in the linked primary study.\n[shin-2014] Mitochondrial MTHFD2L is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase expressed in both adult and embryonic tissues (2014). https://pubmed.ncbi.nlm.nih.gov/24733394/ DOI: 10.1074/jbc.m114.555573","model_system":"Purified recombinant rat protein","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [shin-2014] Mitochondrial MTHFD2L is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase expressed in both adult and embryonic tissues (2014). https://pubmed.ncbi.nlm.nih.gov/24733394/ DOI: 10.1074/jbc.m114.555573","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}