{"id":"2f633afa-b613-5201-8659-b1a6aea28d55","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-afmid-hydrolysis","predicate":"hydrolyzes_to","statement":"Recombinant mouse Afmid hydrolyzed N-formyl-L-kynurenine with a Km of 0.18–0.19 mM, matching native mouse cytosolic enzyme.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"db31ac97-f80c-52bc-a3e8-576c751e9a3f","mechanism_event_label":"A second enzyme removes the formyl group to produce kynurenine.","subject":{"id":"1061b155-e24c-5159-a052-40b3e78bdc00","slug":"mouse-afmid","display_name":"Mouse arylformamidase / Afmid","entity_type_key":"protein"},"object":{"id":"ce72cbd6-7077-5f23-a859-a8000a3f8194","slug":"kynurenine","display_name":"L-Kynurenine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"db31ac97-f80c-52bc-a3e8-576c751e9a3f","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-afmid-hydrolysis-event","event_type":"observed_relationship","label":"A second enzyme removes the formyl group to produce kynurenine.","description":"Recombinant mouse Afmid hydrolyzed N-formyl-L-kynurenine with a Km of 0.18–0.19 mM, matching native mouse cytosolic enzyme.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1061b155-e24c-5159-a052-40b3e78bdc00","slug":"mouse-afmid","display_name":"Mouse arylformamidase / Afmid","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ce72cbd6-7077-5f23-a859-a8000a3f8194","slug":"kynurenine","display_name":"L-Kynurenine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"540784df-850e-5eac-a71c-22556535e471","slug":"n-formylkynurenine","display_name":"N-Formyl-L-kynurenine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse liver enzyme expressed in E. coli.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This experiment is mouse enzyme evidence, not direct human AFMID validation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A second enzyme removes the formyl group to produce kynurenine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cloning, expression, and catalytic triad of recombinant arylformamidase. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15935693/ · DOI 10.1016/j.pep.2005.04.013","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8580eff4-72a1-5935-afb0-6da3f302c4db","evidence_kind":"source_excerpt","locator":"Lines 186-192","start_line":186,"end_line":192,"excerpt":"## tryptophan-afmid-hydrolysis\nA second enzyme removes the formyl group to produce kynurenine.\nRecombinant mouse Afmid hydrolyzed N-formyl-L-kynurenine with a Km of 0.18–0.19 mM, matching native mouse cytosolic enzyme.\nModel: Mouse liver enzyme expressed in E. coli.\nLimitations: This experiment is mouse enzyme evidence, not direct human AFMID validation.\nEvidence access: Primary abstract\nCloning, expression, and catalytic triad of recombinant arylformamidase. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15935693/ · DOI 10.1016/j.pep.2005.04.013","model_system":"Mouse liver enzyme expressed in E. coli.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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