{"id":"2f4e3a4c-2044-5ebf-a586-997371f9281c","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-iron-context","predicate":"context_dependent_observation","statement":"Apigenin had antioxidant effects at lower iron exposure but pro-oxidant effects at higher iron exposure in the tested hepatocyte system.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"90a2041f-9a41-536a-86d7-b4bab4327b26","mechanism_event_label":"Apigenin had antioxidant effects at lower iron exposure but pro-oxidant effects at higher iron exposure in the tested hepatocyte system.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"5e7be3df-4f3e-5165-aada-f6dce57185dc","slug":"api-hepatocyte-lipid-peroxidation","display_name":"Lipid peroxidation in metal-challenged, linolenate-loaded hepatocytes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"90a2041f-9a41-536a-86d7-b4bab4327b26","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-iron-context-event","event_type":"experimental_observation","label":"Apigenin had antioxidant effects at lower iron exposure but pro-oxidant effects at higher iron exposure in the tested hepatocyte system.","description":"### api-iron-context\n\nApigenin had antioxidant effects at lower iron exposure but pro-oxidant effects at higher iron exposure in the tested hepatocyte system.\nThis is an exposure-dependent cell result, not a prediction of dietary iron interactions.\nModel and exposure: Hepatocytes loaded with linolenic acid; metals 20–500 micromolar, flavonoids 10–500 micromolar; species unresolved in accessed abstract.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Anti- and pro-oxidative effects of flavonoids on metal-induced lipid hydroperoxide-dependent lipid peroxidation in cultured hepatocytes loaded with alpha-linolenic acid. | 1999 | https://pubmed.ncbi.nlm.nih.gov/10641726/ | https://doi.org/10.1016/s0891-5849(99)00167-7 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Apigenin with varying iron exposure","sequence_order":0,"notes":""},{"entity":{"id":"5e7be3df-4f3e-5165-aada-f6dce57185dc","slug":"api-hepatocyte-lipid-peroxidation","display_name":"Lipid peroxidation in metal-challenged, linolenate-loaded hepatocytes","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"mixed","sequence_order":1,"notes":""},{"entity":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"role":"varying metal condition","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"Apigenin with varying iron exposure","comparator":"Matched metal-exposed cells without flavonoid","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"varying exposure; matched metal-exposed comparison","comparator":"Matched metal-exposed cells without flavonoid","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Apigenin with varying iron exposure\", \"comparator\": \"Matched metal-exposed cells without flavonoid\", \"endpoint\": \"Apigenin had antioxidant effects at lower iron exposure but pro-oxidant effects at higher iron exposure in the tested hepatocyte system.\", \"effect_direction\": \"mixed\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"apigenin\", \"state\": \"Apigenin with varying iron exposure\"}, {\"entity_slug\": \"iron\", \"state\": \"varying exposure; matched metal-exposed comparison\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Hepatocytes loaded with linolenic acid; metals 20–500 micromolar, flavonoids 10–500 micromolar; species unresolved in accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Apigenin had antioxidant effects at lower iron exposure but pro-oxidant effects at higher iron exposure in the tested hepatocyte system.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Anti- and pro-oxidative effects of flavonoids on metal-induced lipid hydroperoxide-dependent lipid peroxidation in cultured hepatocytes loaded with alpha-linolenic acid. | https://pubmed.ncbi.nlm.nih.gov/10641726/ | https://doi.org/10.1016/s0891-5849(99)00167-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-iron-context; This is an exposure-dependent cell result, not a prediction of dietary iron interactions.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d6e27146-2569-5be2-890f-a62923a4615f","evidence_kind":"source_excerpt","locator":"Lines 920-926","start_line":920,"end_line":926,"excerpt":"### api-iron-context\n\nApigenin had antioxidant effects at lower iron exposure but pro-oxidant effects at higher iron exposure in the tested hepatocyte system.\nThis is an exposure-dependent cell result, not a prediction of dietary iron interactions.\nModel and exposure: Hepatocytes loaded with linolenic acid; metals 20–500 micromolar, flavonoids 10–500 micromolar; species unresolved in accessed abstract.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Anti- and pro-oxidative effects of flavonoids on metal-induced lipid hydroperoxide-dependent lipid peroxidation in cultured hepatocytes loaded with alpha-linolenic acid. | 1999 | https://pubmed.ncbi.nlm.nih.gov/10641726/ | https://doi.org/10.1016/s0891-5849(99)00167-7 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"Hepatocytes loaded with linolenic acid; metals 20–500 micromolar, flavonoids 10–500 micromolar; species unresolved in accessed abstract.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}