{"id":"2f4d4437-b2de-54de-a0e7-b075693b7954","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:slc2a2-knockdown","predicate":"decreases_in_recorded_experiment","statement":"Knocking down SLC2A2 reduced C3G transport in differentiated human Caco-2 monolayers relative to scrambled siRNA.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"2c948f60-907d-5a84-9515-69ecbab56617","mechanism_event_label":"Knocking down SLC2A2 reduced C3G transport in differentiated human Caco-2 monolayers relative to scrambled siRNA.","subject":{"id":"75149db0-02f7-5d32-8c7a-5c111d90d8ee","slug":"cyanidin-3-glucoside","display_name":"Cyanidin 3-O-beta-D-glucopyranoside","entity_type_key":"small_molecule"},"object":{"id":"88f0dd28-59a6-5de9-8045-c3a44de03b53","slug":"human-caco2-c3g-transport","display_name":"C3G transport across differentiated human Caco-2 monolayers","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2c948f60-907d-5a84-9515-69ecbab56617","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:slc2a2-knockdown-event","event_type":"experimental_observation","label":"Knocking down SLC2A2 reduced C3G transport in differentiated human Caco-2 monolayers relative to scrambled siRNA.","description":"**SGLT1 and GLUT2 have experimental support.** In differentiated human Caco-2 monolayers, pharmacological inhibition and separate SGLT1 or GLUT2 siRNA reduced C3G transport. Transport was examined across 10–40 µM C3G and up to 120 minutes; scrambled siRNA provided a comparator. This supports involvement in that epithelial model, not an exclusive absorption route or a measured contribution in the human intestine. Canonical proteins are SLC5A1/SGLT1 and SLC2A2/GLUT2. [Zou et al., 2014](https://pmc.ncbi.nlm.nih.gov/articles/PMC4210911/).","status":"provisional","compartment":null,"participants":[{"entity":{"id":"75149db0-02f7-5d32-8c7a-5c111d90d8ee","slug":"cyanidin-3-glucoside","display_name":"Cyanidin 3-O-beta-D-glucopyranoside","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"C3G exposure with SLC2A2 siRNA","sequence_order":0,"notes":""},{"entity":{"id":"88f0dd28-59a6-5de9-8045-c3a44de03b53","slug":"human-caco2-c3g-transport","display_name":"C3G transport across differentiated human Caco-2 monolayers","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"5a760e20-e07a-5725-8f72-8d4b64e71d6b","slug":"slc2a2","display_name":"Human glucose transporter 2 / SLC2A2 / GLUT2","entity_type_key":"protein"},"role":"knocked-down transporter","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Full text retrieved; relevant methods/results/figure text reviewed. No independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"present","comparator":"C3G exposure with scrambled siRNA","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"cyanidin-3-glucoside","display_name":"Cyanidin 3-O-beta-D-glucopyranoside","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"siRNA knockdown","comparator":"C3G exposure with scrambled siRNA","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"slc2a2","display_name":"Human glucose transporter 2 / SLC2A2 / GLUT2","entity_type_key":"protein"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"C3G exposure with SLC2A2 siRNA\", \"comparator\": \"C3G exposure with scrambled siRNA\", \"endpoint\": \"Knocking down SLC2A2 reduced C3G transport in differentiated human Caco-2 monolayers relative to scrambled siRNA.\", \"effect_direction\": \"decrease\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"slc2a2\", \"state\": \"siRNA knockdown\"}, {\"entity_slug\": \"cyanidin-3-glucoside\", \"state\": \"present\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Differentiated human Caco-2; 10-40 uM C3G transport experiments up to 120 min; siRNA comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Knockdown result, not a wild-type inhibitory action of the transporter. Does not quantify its contribution to human in vivo absorption.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Knocking down SLC2A2 reduced C3G transport in differentiated human Caco-2 monolayers relative to scrambled siRNA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The role of sodium-dependent glucose transporter 1 and glucose transporter 2 in the absorption of cyanidin-3-o-β-glucoside in Caco-2 cells. | 2014 | DOI 10.3390/nu6104165 | PMID 25314643 | https://pubmed.ncbi.nlm.nih.gov/25314643/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC4210911/ | https://doi.org/10.3390/nu6104165","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 38-38; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"3a700213-64d8-501f-84fe-af1090f87087","evidence_kind":"source_excerpt","locator":"Lines 38-38","start_line":38,"end_line":38,"excerpt":"**SGLT1 and GLUT2 have experimental support.** In differentiated human Caco-2 monolayers, pharmacological inhibition and separate SGLT1 or GLUT2 siRNA reduced C3G transport. Transport was examined across 10–40 µM C3G and up to 120 minutes; scrambled siRNA provided a comparator. This supports involvement in that epithelial model, not an exclusive absorption route or a measured contribution in the human intestine. Canonical proteins are SLC5A1/SGLT1 and SLC2A2/GLUT2. [Zou et al., 2014](https://pmc.ncbi.nlm.nih.gov/articles/PMC4210911/).","model_system":"Differentiated human Caco-2; 10-40 uM C3G transport experiments up to 120 min; siRNA comparison.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b08e7f7d-4d34-56d7-a185-2124f8d72b3c","stable_key":"import-35ec55a7-323c-5c28-979e-3bdafe9d5769","title":"Anthocyanins: detailed mechanisms of action (reviewed 4 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"7cff1a47fbd9c625412b84162b1c823004b4162b7c009f9a11d5807fb8e04ef9","revision_id":"1bd4fa42-bfeb-5148-8250-fc363d8c1de0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}