{"id":"2f1c82ad-e3f3-59dd-8364-137bddf43dad","stable_key":"911fb3c7-8cc3-5667-b677-5682fab67648:histidine-skin-flg-processing","predicate":"increases_tested","statement":"Adding L-histidine increased filaggrin monomer formation in the reported human keratinocyte/skin-equivalent experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e3e331b4-933c-599c-a330-46b552f882ef","mechanism_event_label":"More available substrate changed a skin-protein processing readout in culture.","subject":{"id":"44374451-3436-5136-a8ae-5dcc6d8c353b","slug":"histidine","display_name":"L-Histidine","entity_type_key":"small_molecule"},"object":{"id":"ec90da92-1bbd-56ff-86c1-c6a4bac32093","slug":"flg","display_name":"Human filaggrin / FLG","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"e3e331b4-933c-599c-a330-46b552f882ef","stable_key":"911fb3c7-8cc3-5667-b677-5682fab67648:histidine-skin-flg-processing-event","event_type":"observed_relationship","label":"More available substrate changed a skin-protein processing readout in culture.","description":"Adding L-histidine increased filaggrin monomer formation in the reported human keratinocyte/skin-equivalent experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"44374451-3436-5136-a8ae-5dcc6d8c353b","slug":"histidine","display_name":"L-Histidine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ec90da92-1bbd-56ff-86c1-c6a4bac32093","slug":"flg","display_name":"Human filaggrin / FLG","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human keratinocyte differentiation and skin-equivalent models.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Culture response does not prove all eczema reflects histidine shortage or that FLG null variants can be repaired; one author was a director of a patent-owning company.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Histidine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"histidine","display_name":"L-Histidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"More available substrate changed a skin-protein processing readout in culture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Feeding filaggrin: effects of l-histidine supplementation in atopic dermatitis. · 2017 · https://pubmed.ncbi.nlm.nih.gov/29042806/ · DOI 10.2147/CCID.S146760","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"86543881-b6e7-5dbc-9a3e-c987387e3a42","evidence_kind":"source_excerpt","locator":"Lines 410-416","start_line":410,"end_line":416,"excerpt":"## histidine-skin-flg-processing\nMore available substrate changed a skin-protein processing readout in culture.\nAdding L-histidine increased filaggrin monomer formation in the reported human keratinocyte/skin-equivalent experiments.\nModel: Human keratinocyte differentiation and skin-equivalent models.\nLimitations: Culture response does not prove all eczema reflects histidine shortage or that FLG null variants can be repaired; one author was a director of a patent-owning company.\nEvidence access: Primary full text\nFeeding filaggrin: effects of l-histidine supplementation in atopic dermatitis. · 2017 · https://pubmed.ncbi.nlm.nih.gov/29042806/ · DOI 10.2147/CCID.S146760","model_system":"Human keratinocyte differentiation and skin-equivalent models.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ce59e6c9-1213-523b-9d0b-400b7a81cd1c","stable_key":"import-911fb3c7-8cc3-5667-b677-5682fab67648","title":"L-Histidine: supply, catabolism, histamine, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"cb672530ec8b4a4542d0f3957e80ca76bb0449e9f988845b0f7681f623c4ec9b","revision_id":"19a7f26c-d9a1-5411-a81d-025f3d98a452","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}