{"id":"2ef99979-9325-596a-8b33-6b36b123cabd","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-trans-smvt-lipoate","predicate":"mediates_uptake","statement":"Expression of cloned human SLC5A6 in HRPE cells conferred sodium-dependent uptake of lipoate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"94cc08f7-5e3b-54e9-8502-006d1a221d5d","mechanism_event_label":"SMVT carries lipoate into cells using sodium-dependent transport.","subject":{"id":"3bd59e38-e6b1-5223-b4de-faa33835f5bc","slug":"slc5a6","display_name":"Human sodium-dependent multivitamin transporter / SLC5A6","entity_type_key":"protein"},"object":{"id":"7573bab1-f23f-5fbc-9d65-434bd7fa96b8","slug":"cellular-lipoate-uptake","display_name":"Cellular free lipoate uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"94cc08f7-5e3b-54e9-8502-006d1a221d5d","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-trans-smvt-lipoate-event","event_type":"biochemical_relationship","label":"SMVT carries lipoate into cells using sodium-dependent transport.","description":"Expression of cloned human SLC5A6 in HRPE cells conferred sodium-dependent uptake of lipoate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ab091982-3acb-5a87-80fb-85dfe292c1e0","slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"cotransported_ion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3bd59e38-e6b1-5223-b4de-faa33835f5bc","slug":"slc5a6","display_name":"Human sodium-dependent multivitamin transporter / SLC5A6","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7573bab1-f23f-5fbc-9d65-434bd7fa96b8","slug":"cellular-lipoate-uptake","display_name":"Cellular free lipoate uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human SMVT cloned from JAR cells, expressed in human retinal pigment epithelial cells and Xenopus laevis oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Human SMVT cDNA expression; substrate concentrations not specified in the abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Heterologous human-protein experiments establish transport properties; they do not quantify whole-body absorption or nutrient deficiency from supplement competition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens (protein and HRPE expression cells)","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"SMVT carries lipoate into cells using sodium-dependent transport.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-trans-wang1999] Human placental Na+-dependent multivitamin transporter. Cloning, functional expression, gene structure, and chromosomal localization. (1999). https://pubmed.ncbi.nlm.nih.gov/10329687/ DOI: 10.1074/jbc.274.21.14875","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HRPE cell plasma membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0bc07da9-e9b3-5615-b280-ba8ffe1041b3","evidence_kind":"source_excerpt","locator":"Lines 184-195","start_line":184,"end_line":195,"excerpt":"### b5-trans-smvt-lipoate\nExpression of cloned human SLC5A6 in HRPE cells conferred sodium-dependent uptake of lipoate.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: SMVT carries lipoate into cells using sodium-dependent transport.\norganism: Homo sapiens (protein and HRPE expression cells)\ntissue_or_cell_type: HRPE cell plasma membrane\nexperimental_model: Human SMVT cloned from JAR cells, expressed in human retinal pigment epithelial cells and Xenopus laevis oocytes\nlimitations: Heterologous human-protein experiments establish transport properties; they do not quantify whole-body absorption or nutrient deficiency from supplement competition.\nexposure: Human SMVT cDNA expression; substrate concentrations not specified in the abstract.\ncross_nutrient: true\n[b5-trans-wang1999] Human placental Na+-dependent multivitamin transporter. Cloning, functional expression, gene structure, and chromosomal localization. (1999). https://pubmed.ncbi.nlm.nih.gov/10329687/ DOI: 10.1074/jbc.274.21.14875","model_system":"Human SMVT cloned from JAR cells, expressed in human retinal pigment epithelial cells and Xenopus laevis oocytes","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-trans-wang1999] Human placental Na+-dependent multivitamin transporter. Cloning, functional expression, gene structure, and chromosomal localization. (1999). https://pubmed.ncbi.nlm.nih.gov/10329687/ DOI: 10.1074/jbc.274.21.14875","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}