{"id":"2eaf0778-d49f-5213-851d-bb08448c6afb","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-tributyrin-repletion","predicate":"replenishes","statement":"Tributyrin administration replenished colonic butyrate and HIF-related responses in antibiotic-treated mice in the PHD study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e9850d1a-7faa-520f-8beb-7e8613ad7d35","mechanism_event_label":"A glycerol ester supplied butyrate in the depletion experiment.","subject":{"id":"11789041-6065-5870-a2a8-8674b713a3ad","slug":"tributyrin","display_name":"Tributyrin / glycerol tributyrate","entity_type_key":"lipid"},"object":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"e9850d1a-7faa-520f-8beb-7e8613ad7d35","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-tributyrin-repletion-event","event_type":"observed_relationship","label":"A glycerol ester supplied butyrate in the depletion experiment.","description":"Tributyrin administration replenished colonic butyrate and HIF-related responses in antibiotic-treated mice in the PHD study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"11789041-6065-5870-a2a8-8674b713a3ad","slug":"tributyrin","display_name":"Tributyrin / glycerol tributyrate","entity_type_key":"lipid"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e52249c8-a1b7-5521-ba46-f4e4656bdd2e","slug":"mouse-butyrate-hif-stabilization","display_name":"HIF stabilization in mouse colon after butyrate repletion","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text, Figure 5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Antibiotic-treated mice; tributyrin reconstitution.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Tributyrin and free sodium butyrate are different preparations; this does not establish equal release sites or human doses.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Butyrate collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A glycerol ester supplied butyrate in the depletion experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Microbiota-derived butyrate is an endogenous HIF prolyl hydroxylase inhibitor. · 2021 · https://pubmed.ncbi.nlm.nih.gov/34190032/ · DOI 10.1080/19490976.2021.1938380","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7fd25853-f1a5-5b72-8c5d-56f6ba5099ad","evidence_kind":"source_excerpt","locator":"Lines 158-164","start_line":158,"end_line":164,"excerpt":"## butyrate-tributyrin-repletion\nA glycerol ester supplied butyrate in the depletion experiment.\nTributyrin administration replenished colonic butyrate and HIF-related responses in antibiotic-treated mice in the PHD study.\nModel: Antibiotic-treated mice; tributyrin reconstitution.\nLimitations: Tributyrin and free sodium butyrate are different preparations; this does not establish equal release sites or human doses.\nEvidence access: Primary full text, Figure 5\nMicrobiota-derived butyrate is an endogenous HIF prolyl hydroxylase inhibitor. · 2021 · https://pubmed.ncbi.nlm.nih.gov/34190032/ · DOI 10.1080/19490976.2021.1938380","model_system":"Antibiotic-treated mice; tributyrin reconstitution.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"33205bb8-dcbe-5d2c-afca-86075d7748d1","stable_key":"import-104835a3-b891-5dc4-a6bb-42ab7c6f96c4","title":"Butyrate: microbial production, fuel use, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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