{"id":"2e483393-9590-5da7-8913-7cd2e2e1300e","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-tryptamine-transit","predicate":"microbial_production_accelerates","statement":"Colonizing germ-free mice with B. thetaiotaomicron engineered to produce tryptamine accelerated gastrointestinal transit.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b37333b8-acb4-563d-b6b3-369c38fdb647","mechanism_event_label":"Changing microbial production changed movement through the gut.","subject":{"id":"e009ad82-c5b9-5dba-96dd-b7e85060da2c","slug":"tryptamine","display_name":"Tryptamine","entity_type_key":"small_molecule"},"object":{"id":"145e4599-cb23-5872-851c-3a4f12314e83","slug":"mouse-tryptamine-transit","display_name":"Mouse gastrointestinal transit after microbial tryptamine","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b37333b8-acb4-563d-b6b3-369c38fdb647","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-tryptamine-transit-event","event_type":"observed_relationship","label":"Changing microbial production changed movement through the gut.","description":"Colonizing germ-free mice with B. thetaiotaomicron engineered to produce tryptamine accelerated gastrointestinal transit.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e009ad82-c5b9-5dba-96dd-b7e85060da2c","slug":"tryptamine","display_name":"Tryptamine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"145e4599-cb23-5872-851c-3a4f12314e83","slug":"mouse-tryptamine-transit","display_name":"Mouse gastrointestinal transit after microbial tryptamine","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"fd074f31-c694-5703-9237-0544bf024341","slug":"mouse-htr4","display_name":"Mouse serotonin receptor 4 / Htr4","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Engineered bacterium in gnotobiotic mice.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not evidence that tryptophan supplements have the same effect in humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Changing microbial production changed movement through the gut.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Gut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29902441/ · DOI 10.1016/j.chom.2018.05.004","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9b8606bf-aca5-5783-8233-9c706a66be75","evidence_kind":"source_excerpt","locator":"Lines 578-584","start_line":578,"end_line":584,"excerpt":"## tryptophan-tryptamine-transit\nChanging microbial production changed movement through the gut.\nColonizing germ-free mice with B. thetaiotaomicron engineered to produce tryptamine accelerated gastrointestinal transit.\nModel: Engineered bacterium in gnotobiotic mice.\nLimitations: Not evidence that tryptophan supplements have the same effect in humans.\nEvidence access: Primary abstract\nGut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29902441/ · DOI 10.1016/j.chom.2018.05.004","model_system":"Engineered bacterium in gnotobiotic mice.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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