{"id":"2e358ab4-0b1f-5d16-be2a-1cf2c7f1bc6a","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-cpg","predicate":"reduces","statement":"At 5–50 micromolar for 12–144 hours, EGCG reduced methylation-specific signals at p16, RARbeta, MGMT and hMLH1 promoters in KYSE 510 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"29887864-27eb-514c-b20e-782db82441e9","mechanism_event_label":"Some silenced cancer-cell genes showed changes in promoter methylation.","subject":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"object":{"id":"db19a24e-7048-5bbc-9679-68717ce9c69a","slug":"cancer-promoter-cpg-methylation","display_name":"Promoter CpG methylation in specified cancer cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"29887864-27eb-514c-b20e-782db82441e9","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-cpg-event","event_type":"observed_relationship","label":"Some silenced cancer-cell genes showed changes in promoter methylation.","description":"At 5–50 micromolar for 12–144 hours, EGCG reduced methylation-specific signals at p16, RARbeta, MGMT and hMLH1 promoters in KYSE 510 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"db19a24e-7048-5bbc-9679-68717ce9c69a","slug":"cancer-promoter-cpg-methylation","display_name":"Promoter CpG methylation in specified cancer cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human esophageal cancer cells; PCR methylation assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not a universal epigenome reset or evidence that demethylation is always beneficial.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"EGCG collection; comparator and shared-pathway records retain their actual intervention.","comparator":null,"unit":null,"notes":"","entity":{"slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Some silenced cancer-cell genes showed changes in promoter methylation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Tea polyphenol (-)-epigallocatechin-3-gallate inhibits DNA methyltransferase and reactivates methylation-silenced genes in cancer cell lines. · 2003 · https://pubmed.ncbi.nlm.nih.gov/14633667/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f5826a61-a6ab-55f8-9749-4ea4bdcc37ef","evidence_kind":"source_excerpt","locator":"Lines 260-266","start_line":260,"end_line":266,"excerpt":"## egcg-cpg\nSome silenced cancer-cell genes showed changes in promoter methylation.\nAt 5–50 micromolar for 12–144 hours, EGCG reduced methylation-specific signals at p16, RARbeta, MGMT and hMLH1 promoters in KYSE 510 cells.\nModel: Human esophageal cancer cells; PCR methylation assays.\nLimitations: Not a universal epigenome reset or evidence that demethylation is always beneficial.\nEvidence access: primary abstract.\nTea polyphenol (-)-epigallocatechin-3-gallate inhibits DNA methyltransferase and reactivates methylation-silenced genes in cancer cell lines. · 2003 · https://pubmed.ncbi.nlm.nih.gov/14633667/","model_system":"Human esophageal cancer cells; PCR methylation assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Primary-abstract paraphrase; no full-text methods verification claimed.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1f6d09e8-3aae-5c41-9ea7-15d0d1cab72d","stable_key":"import-ec987f3e-0b3c-55cc-b304-c703a738e35d","title":"EGCG: receptor signaling, metabolism, nutrient interactions and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b101c8d4d96ee03ff97eb1922c7bb0f96943295af473fdc3ef98b0dbd62fe8c7","revision_id":"e19bc5b2-ee3c-5f67-b5c5-9dc3b7d5f850","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}