{"id":"2e333805-0ad2-5063-8a1c-11ceeddc46c4","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-mocs2a-v7f","predicate":"weakens_interaction_with","statement":"MOCS2A Val7Phe weakened its interaction with MOCS2B in reconstituted human MPT synthase.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2bce404e-5214-525b-af1e-6f5c55dd276d","mechanism_event_label":"A damaged sulfur-carrier subunit may no longer fit its partner.","subject":{"id":"085a39b7-511d-549e-99fb-242130b2eeba","slug":"mocs2a-v7f","display_name":"Human MOCS2A p.Val7Phe","entity_type_key":"protein_state"},"object":{"id":"09113aa9-5974-5c1b-ad62-9fb8c59ede47","slug":"mocs2b","display_name":"Human molybdopterin synthase catalytic subunit / MOCS2B","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"2bce404e-5214-525b-af1e-6f5c55dd276d","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-mocs2a-v7f-event","event_type":"biochemical_relationship","label":"A damaged sulfur-carrier subunit may no longer fit its partner.","description":"MOCS2A Val7Phe weakened its interaction with MOCS2B in reconstituted human MPT synthase.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"085a39b7-511d-549e-99fb-242130b2eeba","slug":"mocs2a-v7f","display_name":"Human MOCS2A p.Val7Phe","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"09113aa9-5974-5c1b-ad62-9fb8c59ede47","slug":"mocs2b","display_name":"Human molybdopterin synthase catalytic subunit / MOCS2B","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/12732628.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"154da4e4528674556bef92dfe50f098cfd9ae715c06ea01fc17d64c64b8aa319\", \"start_char\": 0, \"end_char\": 1322, \"text_sha256\": \"154da4e4528674556bef92dfe50f098cfd9ae715c06ea01fc17d64c64b8aa319\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human MPT synthase and patient-derived point mutants","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Precursor Z/cPMP conversion assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"In-vitro assembly and kinetic defects; severity comparisons refer to specific patients, not a universal hierarchy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human proteins expressed in Escherichia coli","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A damaged sulfur-carrier subunit may no longer fit its partner.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p12732628] Mechanistic studies of human molybdopterin synthase reaction and characterization of mutants identified in group B patients of molybdenum cofactor deficiency. 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