{"id":"2e1d5935-d27e-594b-bd3a-8904fae39b07","stable_key":"0ad8610d-d575-5870-b7cd-763a9f750783:copper-pde3b-camp-lipolysis","predicate":"copper_inhibition_enhances","statement":"Copper-dependent PDE3B inhibition increased cAMP signaling and promoted lipolysis in 3T3-L1 adipocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3ebe5591-ad00-5b3d-8b17-0012fcb52a7d","mechanism_event_label":"The prolonged signal encouraged fat breakdown in these cells.","subject":{"id":"76a46ee5-623f-558f-be84-327fb74dfb08","slug":"mouse-pde3b","display_name":"Mouse cGMP-inhibited phosphodiesterase Pde3b","entity_type_key":"protein"},"object":{"id":"609e2bca-5c38-55d5-a4fc-a65e72f98427","slug":"mouse-adipocyte-lipolysis","display_name":"Mouse adipocyte lipolysis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3ebe5591-ad00-5b3d-8b17-0012fcb52a7d","stable_key":"0ad8610d-d575-5870-b7cd-763a9f750783:copper-pde3b-camp-lipolysis-event","event_type":"biochemical_relationship","label":"The prolonged signal encouraged fat breakdown in these cells.","description":"Copper-dependent PDE3B inhibition increased cAMP signaling and promoted lipolysis in 3T3-L1 adipocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ad0ccd02-b864-5373-b21e-85aa6d912f73","slug":"intracellular-camp-content","display_name":"Intracellular cyclic AMP content","entity_type_key":"cellular_process"},"role":"signaling intermediate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"76a46ee5-623f-558f-be84-327fb74dfb08","slug":"mouse-pde3b","display_name":"Mouse cGMP-inhibited phosphodiesterase Pde3b","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"609e2bca-5c38-55d5-a4fc-a65e72f98427","slug":"mouse-adipocyte-lipolysis","display_name":"Mouse adipocyte lipolysis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/copper-research/27272565.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f8b7e13cc6ef608ddb589779fa85430ca530508013e710f380f4020c3f2632c7\", \"start_char\": 0, \"end_char\": 1018, \"text_sha256\": \"f8b7e13cc6ef608ddb589779fa85430ca530508013e710f380f4020c3f2632c7\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Copper manipulation, purified Pde3b assays and adipocyte signaling","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Copper perturbation and Pde3b cysteine mutations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This cellular signaling result is not a human weight-loss trial. Copper toxicity and systemic distribution are not captured by increasing one cell-culture signal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Copper research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"copper","display_name":"Copper","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse proteins and 3T3-L1 adipocytes; Atp7b mutant mouse context","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The prolonged signal encouraged fat breakdown in these cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[copper-p27272565] Copper regulates cyclic-AMP-dependent lipolysis. (2016). https://pubmed.ncbi.nlm.nih.gov/27272565/ DOI: 10.1038/nchembio.2098","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Adipocytes and purified enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cb9f34a2-9ca9-5aac-9c1c-c012b01296bf","evidence_kind":"source_excerpt","locator":"Lines 1209-1220","start_line":1209,"end_line":1220,"excerpt":"### copper-pde3b-camp-lipolysis\nCopper-dependent PDE3B inhibition increased cAMP signaling and promoted lipolysis in 3T3-L1 adipocytes.\nCondition category: normal\nnutrient_topic: Copper research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The prolonged signal encouraged fat breakdown in these cells.\norganism: Mouse proteins and 3T3-L1 adipocytes; Atp7b mutant mouse context\ntissue_or_cell_type: Adipocytes and purified enzyme\nexperimental_model: Copper manipulation, purified Pde3b assays and adipocyte signaling\nlimitations: This cellular signaling result is not a human weight-loss trial. Copper toxicity and systemic distribution are not captured by increasing one cell-culture signal.\nexposure: Copper perturbation and Pde3b cysteine mutations\nevidence_span: {\"source_cache\": \"artifacts/copper-research/27272565.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f8b7e13cc6ef608ddb589779fa85430ca530508013e710f380f4020c3f2632c7\", \"start_char\": 0, \"end_char\": 1018, \"text_sha256\": \"f8b7e13cc6ef608ddb589779fa85430ca530508013e710f380f4020c3f2632c7\"}\n[copper-p27272565] Copper regulates cyclic-AMP-dependent lipolysis. (2016). https://pubmed.ncbi.nlm.nih.gov/27272565/ DOI: 10.1038/nchembio.2098","model_system":"Copper manipulation, purified Pde3b assays and adipocyte signaling","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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