{"id":"2e1b1d69-683f-503f-bc6c-f30eb2a78e63","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-topoisomerase","predicate":"inhibits","statement":"Fisetin inhibited human topoisomerase II-alpha in an isolated enzyme assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"b5c416b6-7bf2-5b31-8f68-907cdd2b736e","mechanism_event_label":"DNA-handling machinery is another experimental target.","subject":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"object":{"id":"1a919885-9dcf-5ece-a37f-31ee4d489ab5","slug":"top2a","display_name":"Human DNA topoisomerase II alpha / TOP2A","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"b5c416b6-7bf2-5b31-8f68-907cdd2b736e","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-topoisomerase-event","event_type":"observed_relationship","label":"DNA-handling machinery is another experimental target.","description":"Fisetin inhibited human topoisomerase II-alpha in an isolated enzyme assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1a919885-9dcf-5ece-a37f-31ee4d489ab5","slug":"top2a","display_name":"Human DNA topoisomerase II alpha / TOP2A","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Biochemical confirmation accompanying human-cell genotoxicity testing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not quantify risk from ordinary dietary intake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"DNA-handling machinery is another experimental target.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Chromosomal malsegregation and micronucleus induction in vitro by the DNA topoisomerase II inhibitor fisetin. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15781213/ · DOI 10.1016/j.mrgentox.2005.01.002","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ba7fc49f-7443-5a08-9265-e921d79c8136","evidence_kind":"source_excerpt","locator":"Lines 480-486","start_line":480,"end_line":486,"excerpt":"## fisetin-topoisomerase\nDNA-handling machinery is another experimental target.\nFisetin inhibited human topoisomerase II-alpha in an isolated enzyme assay.\nModel: Biochemical confirmation accompanying human-cell genotoxicity testing.\nLimitations: Does not quantify risk from ordinary dietary intake.\nEvidence access: Primary abstract\nChromosomal malsegregation and micronucleus induction in vitro by the DNA topoisomerase II inhibitor fisetin. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15781213/ · DOI 10.1016/j.mrgentox.2005.01.002","model_system":"Biochemical confirmation accompanying human-cell genotoxicity testing.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c30e0764cd34bd062cfcbfaf32357ef29dfda30e7d4153c545178140f5054e09","revision_id":"40ff52c0-f4b9-5820-8abb-ff002c6364a4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}