{"id":"2e198445-d55e-51c0-8e78-956b54e50503","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-fabri-camp","predicate":"restores-response-under-low-substrate-conditions","statement":"Adding 25(OH)D3 to low-vitamin-D serum restored IFN-gamma -induced antimicrobial peptide expression.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"cbbad19e-1283-5969-8e2b-552257ace1b1","mechanism_event_label":"Adding precursor restored the antimicrobial gene response.","subject":{"id":"6bd73bd2-1f0a-5caa-ba69-7ce1b75364bb","slug":"calcifediol","display_name":"Calcifediol / 25-hydroxyvitamin D3","entity_type_key":"small_molecule"},"object":{"id":"24a49e8d-7865-5e47-92a0-0ffadd48cb24","slug":"human-camp-mrna","display_name":"Human CAMP mRNA","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"cbbad19e-1283-5969-8e2b-552257ace1b1","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-fabri-camp-event","event_type":"biochemical_relationship","label":"Adding precursor restored the antimicrobial gene response.","description":"Adding 25(OH)D3 to low-vitamin-D serum restored IFN-gamma -induced antimicrobial peptide expression.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8b483912-bed0-50b7-a9b3-ec45ce5156da","slug":"interferon-gamma","display_name":"Interferon gamma","entity_type_key":"protein"},"role":"stimulus","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a1d0238c-7529-5175-b5f9-71dc9bc61ed5","slug":"cyp27b1","display_name":"Vitamin D 1-alpha-hydroxylase / CYP27B1","entity_type_key":"protein"},"role":"activation-pathway","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d725020b-bacc-5e90-b370-cda246025c91","slug":"vdr","display_name":"Vitamin D receptor / VDR","entity_type_key":"protein"},"role":"receptor-pathway","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6bd73bd2-1f0a-5caa-ba69-7ce1b75364bb","slug":"calcifediol","display_name":"Calcifediol / 25-hydroxyvitamin D3","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"24a49e8d-7865-5e47-92a0-0ffadd48cb24","slug":"human-camp-mrna","display_name":"Human CAMP mRNA","entity_type_key":"rna"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human macrophage IFN-gamma experiments with sera of differing 25OHD content","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"IFN-gamma -stimulated human macrophages; low-vitamin-D serum with 25(OH)D3 add-back; dose/time not recovered from abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cultured human macrophages and selected serum pools; no universal serum threshold or clinical tuberculosis cure inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Adding precursor restored the antimicrobial gene response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[fabri2011] Vitamin D is required for IFN-gamma-mediated antimicrobial activity of human macrophages. (2011). https://pubmed.ncbi.nlm.nih.gov/21998409/ DOI: 10.1126/scitranslmed.3003045","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human macrophages","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"36e53b07-4c22-50b9-8989-5a001e0f24d0","evidence_kind":"source_excerpt","locator":"Lines 1035-1046","start_line":1035,"end_line":1046,"excerpt":"### vd-fabri-camp\nAdding 25(OH)D3 to low-vitamin-D serum restored IFN-gamma -induced antimicrobial peptide expression.\nCondition category: nutrient_deficiency\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Adding precursor restored the antimicrobial gene response.\norganism: Homo sapiens\ntissue_or_cell_type: Human macrophages\nexperimental_model: Human macrophage IFN-gamma experiments with sera of differing 25OHD content\nlimitations: Cultured human macrophages and selected serum pools; no universal serum threshold or clinical tuberculosis cure inferred.\nexposure: IFN-gamma -stimulated human macrophages; low-vitamin-D serum with 25(OH)D3 add-back; dose/time not recovered from abstract.\ncross_nutrient: false\n[fabri2011] Vitamin D is required for IFN-gamma-mediated antimicrobial activity of human macrophages. 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