{"id":"2dfb8b77-d67b-5593-8cea-ad5b9b2d1632","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:nr-rat-rescue","predicate":"increases","statement":"NR co-treatment opposed the impaired FAO-associated respiration in the 3-TYP rat-cell experiment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"0cd293e0-9617-5f24-a5e8-2c840f920f42","mechanism_event_label":"nr rat rescue","subject":{"id":"7bb72d0e-e20d-51bc-b88b-7ff29dc2ad4a","slug":"nicotinamide-riboside","display_name":"Nicotinamide riboside","entity_type_key":"small_molecule"},"object":{"id":"6314b700-44b5-5993-aa8f-a90e13a25f02","slug":"rat-cardiac-fao","display_name":"Rat cardiac fatty-acid oxidation capacity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0cd293e0-9617-5f24-a5e8-2c840f920f42","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:nr-rat-rescue","event_type":"increases","label":"nr rat rescue","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7bb72d0e-e20d-51bc-b88b-7ff29dc2ad4a","slug":"nicotinamide-riboside","display_name":"Nicotinamide riboside","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6314b700-44b5-5993-aa8f-a90e13a25f02","slug":"rat-cardiac-fao","display_name":"Rat cardiac fatty-acid oxidation capacity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0769c420-b827-5567-986f-19b0c2ecefd6","slug":"3-typ","display_name":"3-TYP experimental SIRT3 inhibitor","entity_type_key":"drug"},"role":"co-exposure","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"Mitochondria or cell lysate, depending on assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Compound concentration not established in reviewed text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Treatment endpoint timing not established in reviewed text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"rat-cardiac-fao","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Figure 2A–G","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Pharmacological 3-TYP experiment; NR co-treatment where stated","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"nicotinamide-riboside","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. The figure tests 3-TYP plus NR; do not mislabel it as an NR-alone dose-response experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Topical cross-reference only; no inheritance of another actor's effects.","comparator":null,"unit":null,"notes":"","entity":{"slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rattus norvegicus host cells; construct species may be unspecified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"NR co-treatment opposed the impaired FAO-associated respiration in the 3-TYP rat-cell experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"[{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 23, \"text_sha256\": \"08f11746524e975c9e1a5e3d53de363a6762891bc05afffbd711f347867ac842\", \"xml_element_id\": \"Par10\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 25, \"text_sha256\": \"1814242d6194ed4e0b3ab78287e20f85b6bc243051183d0f24d9034c3a930257\", \"xml_element_id\": null}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-026-70703-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"sample_size","value_text":"4 respiratory replicates; 6 staining replicates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Neonatal rat ventricular cardiomyocytes (NRVCMs)","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f60ec7b8-1760-5243-97af-8b76d5826e67","evidence_kind":"source_excerpt","locator":"Lines 623-638","start_line":623,"end_line":638,"excerpt":"NR co-treatment opposed the impaired FAO-associated respiration in the 3-TYP rat-cell experiment.\norganism: Rattus norvegicus host cells; construct species may be unspecified\ntissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs)\nexperimental_model: Pharmacological 3-TYP experiment; NR co-treatment where stated\ncompartment: Mitochondria or cell lysate, depending on assay\ndose: Compound concentration not established in reviewed text\nduration: Treatment endpoint timing not established in reviewed text\nprimary_references: https://doi.org/10.1038/s41467-026-70703-w\naccess_level: full_text_and_supplement_review\nevidence_location: Figure 2A–G\nendpoint: rat-cardiac-fao\nexposure: nicotinamide-riboside\nlimitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. The figure tests 3-TYP plus NR; do not mislabel it as an NR-alone dose-response experiment.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 23, \"text_sha256\": \"08f11746524e975c9e1a5e3d53de363a6762891bc05afffbd711f347867ac842\", \"xml_element_id\": \"Par10\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 25, \"text_sha256\": \"1814242d6194ed4e0b3ab78287e20f85b6bc243051183d0f24d9034c3a930257\", \"xml_element_id\": null}]\nplain_language: NR co-treatment opposed the impaired FAO-associated respiration in the 3-TYP rat-cell experiment.\nsample_size: 4 respiratory replicates; 6 staining replicates","model_system":"Pharmacological 3-TYP experiment; NR co-treatment where stated","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. Exact primary locators and access limits retained in contexts.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ee72956d-4fd4-58d3-9a94-e173bb1e3282","stable_key":"import-c156003d-8cdf-5259-8561-9803795ecc82","title":"DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence","document_type":"imported_text","citation_label":"AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation.","file_path":"","sha256":"159e5472c8b1d104625f9c648dc9ee24dcd5fe102109fd960bba50e9ba9de954","revision_id":"5ca6b1f1-fd75-5f8d-ba2b-0215fbf387c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}