{"id":"2dc3e927-1091-549e-a956-86706662d64d","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:lysine-intake-indicator-oxidation","predicate":"increasing_limiting_intake_decreases","statement":"In seven men receiving graded lysine intakes, indicator phenylalanine oxidation fell until a breakpoint and then remained approximately constant.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4dacf569-03b2-585d-8b06-26d92302fd9e","mechanism_event_label":"When lysine was limiting, less of another amino acid was retained; providing more lysine changed that metabolic readout up to a plateau.","subject":{"id":"5fff6664-209e-5c35-b88e-ac15c1c5fbc0","slug":"l-lysine","display_name":"L-Lysine","entity_type_key":"small_molecule"},"object":{"id":"d0d10dcf-4d0c-5c41-a019-4bb4f2af1051","slug":"phenylalanine-oxidation","display_name":"Phenylalanine oxidation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4dacf569-03b2-585d-8b06-26d92302fd9e","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:lysine-intake-indicator-oxidation-event","event_type":"observed_intervention","label":"When lysine was limiting, less of another amino acid was retained; providing more lysine changed that metabolic readout up to a plateau.","description":"In seven men receiving graded lysine intakes, indicator phenylalanine oxidation fell until a breakpoint and then remained approximately constant.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5fff6664-209e-5c35-b88e-ac15c1c5fbc0","slug":"l-lysine","display_name":"L-Lysine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d0d10dcf-4d0c-5c41-a019-4bb4f2af1051","slug":"phenylalanine-oxidation","display_name":"Phenylalanine oxidation","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Controlled isotope feeding experiment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Small male sample; surrogate measure; plateau is not evidence that extra lysine beyond adequacy builds more muscle.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"When lysine was limiting, less of another amino acid was retained; providing more lysine changed that metabolic readout up to a plateau.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zello1993] Dietary lysine requirement of young adult males determined by oxidation of L-[1-13C]phenylalanine (1993). https://pubmed.ncbi.nlm.nih.gov/8476044/ DOI: 10.1152/ajpendo.1993.264.4.E677","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Whole-body amino-acid metabolism","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"d78276e5-02de-5d19-a277-2cee7eeeac02","evidence_kind":"source_excerpt","locator":"Lines 638-646","start_line":638,"end_line":646,"excerpt":"### lysine-intake-indicator-oxidation\nIn seven men receiving graded lysine intakes, indicator phenylalanine oxidation fell until a breakpoint and then remained approximately constant.\nPlain language: When lysine was limiting, less of another amino acid was retained; providing more lysine changed that metabolic readout up to a plateau.\nCondition category: nutrient_deficiency\norganism: Homo sapiens\ntissue_or_cell_type: Whole-body amino-acid metabolism\nexperimental_model: Controlled isotope feeding experiment\nlimitations: Small male sample; surrogate measure; plateau is not evidence that extra lysine beyond adequacy builds more muscle.\n[zello1993] Dietary lysine requirement of young adult males determined by oxidation of L-[1-13C]phenylalanine (1993). https://pubmed.ncbi.nlm.nih.gov/8476044/ DOI: 10.1152/ajpendo.1993.264.4.E677","model_system":"Controlled isotope feeding experiment","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [zello1993] Dietary lysine requirement of young adult males determined by oxidation of L-[1-13C]phenylalanine (1993). https://pubmed.ncbi.nlm.nih.gov/8476044/ DOI: 10.1152/ajpendo.1993.264.4.E677","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}