{"id":"2dae9b4f-3479-52d5-9f91-cfa1aee27287","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:a-vision-rdh5-oxidation","predicate":"produces","statement":"Bovine RDH5 expressed in COS cells oxidized 11-cis-retinol using NAD+; NADP did not support the tested activity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"5646de18-a3db-5900-9e79-9e5c1458e2ea","mechanism_event_label":"RDH5 oxidizes the cis alcohol into the visual chromophore.","subject":{"id":"fec3fe5d-a187-5e6e-bc11-86ad2743a893","slug":"rdh5","display_name":"11-cis-retinol dehydrogenase / RDH5","entity_type_key":"protein"},"object":{"id":"2df0d238-71af-5f0b-8359-cef18235c4af","slug":"11-cis-retinal","display_name":"11-cis-retinal","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"5646de18-a3db-5900-9e79-9e5c1458e2ea","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:a-vision-rdh5-oxidation-event","event_type":"biochemical_relationship","label":"RDH5 oxidizes the cis alcohol into the visual chromophore.","description":"Bovine RDH5 expressed in COS cells oxidized 11-cis-retinol using NAD+; NADP did not support the tested activity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4a978267-139b-518d-bdc6-e16cda6f5225","slug":"11-cis-retinol","display_name":"11-cis-retinol","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"oxidizing cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"be6e4670-953f-5a4e-97da-1e536a54e0e4","slug":"nadp-plus","display_name":"NADP+","entity_type_key":"small_molecule"},"role":"ineffective tested cofactor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"fec3fe5d-a187-5e6e-bc11-86ad2743a893","slug":"rdh5","display_name":"11-cis-retinol dehydrogenase / RDH5","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"2df0d238-71af-5f0b-8359-cef18235c4af","slug":"11-cis-retinal","display_name":"11-cis-retinal","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Recombinant p32/RDH5 activity assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cofactor specificity is biochemical; no niacin-deficiency phenotype was tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Bos taurus protein; COS cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"RDH5 oxidizes the cis alcohol into the visual chromophore.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[simon-1995] The retinal pigment epithelial-specific 11-cis retinol dehydrogenase belongs to the family of short chain alcohol dehydrogenases (1995). https://pubmed.ncbi.nlm.nih.gov/7836368/ DOI: 10.1074/jbc.270.3.1107","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"RPE-derived enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"02a5cef5-2a78-581c-ae5f-b3f533fd5130","evidence_kind":"source_excerpt","locator":"Lines 723-732","start_line":723,"end_line":732,"excerpt":"### a-vision-rdh5-oxidation\nBovine RDH5 expressed in COS cells oxidized 11-cis-retinol using NAD+; NADP did not support the tested activity.\nCondition category: normal\nnutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: RDH5 oxidizes the cis alcohol into the visual chromophore.\norganism: Bos taurus protein; COS cells\ntissue_or_cell_type: RPE-derived enzyme\nexperimental_model: Recombinant p32/RDH5 activity assay\nlimitations: Cofactor specificity is biochemical; no niacin-deficiency phenotype was tested.\n[simon-1995] The retinal pigment epithelial-specific 11-cis retinol dehydrogenase belongs to the family of short chain alcohol dehydrogenases (1995). https://pubmed.ncbi.nlm.nih.gov/7836368/ DOI: 10.1074/jbc.270.3.1107","model_system":"Recombinant p32/RDH5 activity assay","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [simon-1995] The retinal pigment epithelial-specific 11-cis retinol dehydrogenase belongs to the family of short chain alcohol dehydrogenases (1995). https://pubmed.ncbi.nlm.nih.gov/7836368/ DOI: 10.1074/jbc.270.3.1107","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0c5474e2-be48-547b-b287-9c07a59c9ff1","stable_key":"import-dff9f743-3766-5f20-a71e-d6d2bd5bfb6f","title":"Vitamin A: forms, mechanisms, deficiency and excess (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b217224b262abbd77d6a0c8c9beefff9c022fae4d31e2a5273915d769b9a6546","revision_id":"61008909-871f-575c-b5e2-21542689ea3c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}