{"id":"2d8d704e-4778-5d50-accd-435f64232cc7","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-rat-iron-brush-border-uptake","predicate":"supports","statement":"Brush-border membrane vesicles from riboflavin-deficient rats took up less iron than identically prepared control vesicles.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"8785dd43-91e7-5cf8-9f3a-8735d9fe2eaa","mechanism_event_label":"The deficiency affected iron entry at the intestinal surface.","subject":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"object":{"id":"4429d8ae-d732-5f5e-acc1-2c872e23da62","slug":"nonheme-iron-absorption","display_name":"Nonheme iron absorption","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8785dd43-91e7-5cf8-9f3a-8735d9fe2eaa","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-rat-iron-brush-border-uptake-event","event_type":"biochemical_relationship","label":"The deficiency affected iron entry at the intestinal surface.","description":"Brush-border membrane vesicles from riboflavin-deficient rats took up less iron than identically prepared control vesicles.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"role":"transported nutrient","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4429d8ae-d732-5f5e-acc1-2c872e23da62","slug":"nonheme-iron-absorption","display_name":"Nonheme iron absorption","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"B2 deficiency impairs an iron-uptake process in this rat model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Dietary riboflavin-deficient male rats versus age-/weight-matched controls; intragastric 59Fe and mucosal brush-border vesicles.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Dietary riboflavin deficiency","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No specific transporter or ferrireductase was identified; the result must not be assigned to DMT1, DCYTB or ferritin release without evidence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The deficiency affected iron entry at the intestinal surface.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[butler1993] Comparison of changes in the uptake and mucosal processing of iron in riboflavin-deficient rats. (1993). https://pubmed.ncbi.nlm.nih.gov/8358336/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Intestinal brush-border membrane vesicles","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"0dfc2edc-4b6f-57c8-b8bc-cd077daac90c","evidence_kind":"source_excerpt","locator":"Lines 1402-1414","start_line":1402,"end_line":1414,"excerpt":"### b2-rat-iron-brush-border-uptake\nBrush-border membrane vesicles from riboflavin-deficient rats took up less iron than identically prepared control vesicles.\nCondition category: nutrient_deficiency\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The deficiency affected iron entry at the intestinal surface.\norganism: Rattus norvegicus\ntissue_or_cell_type: Intestinal brush-border membrane vesicles\nexperimental_model: Dietary riboflavin-deficient male rats versus age-/weight-matched controls; intragastric 59Fe and mucosal brush-border vesicles.\nlimitations: No specific transporter or ferrireductase was identified; the result must not be assigned to DMT1, DCYTB or ferritin release without evidence.\nexposure: Dietary riboflavin deficiency\ncross_nutrient: B2 deficiency impairs an iron-uptake process in this rat model.\nevidence_location: Abstract\n[butler1993] Comparison of changes in the uptake and mucosal processing of iron in riboflavin-deficient rats. (1993). https://pubmed.ncbi.nlm.nih.gov/8358336/","model_system":"Dietary riboflavin-deficient male rats versus age-/weight-matched controls; intragastric 59Fe and mucosal brush-border vesicles.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [butler1993] Comparison of changes in the uptake and mucosal processing of iron in riboflavin-deficient rats. (1993). https://pubmed.ncbi.nlm.nih.gov/8358336/","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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