{"id":"2d73f77f-0615-515b-ac7a-4050a8cc99b7","stable_key":"4d8e4781-3878-5818-8d0a-7dbd6e024555:chromium-rat-pi3k-activity","predicate":"enhances_activity_of","statement":"Chromium picolinate increased insulin-stimulated PI3K activity in muscle from the obese rats without increasing PI3K protein abundance.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"7514607a-4108-5389-b41f-4f69bfa8770f","mechanism_event_label":"Another downstream insulin-signaling step became more active.","subject":{"id":"89d5d39c-2663-5e0c-8eae-562a5b25a47d","slug":"chromium-iii-picolinate","display_name":"Chromium(III) picolinate / CrPic","entity_type_key":"chemical_species"},"object":{"id":"fe056b29-565f-5535-a9cd-6e4e2c0d7c5f","slug":"rat-pi3k-complexes","display_name":"Rat phosphatidylinositol 3-kinase complexes (isoform unresolved)","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"7514607a-4108-5389-b41f-4f69bfa8770f","stable_key":"4d8e4781-3878-5818-8d0a-7dbd6e024555:chromium-rat-pi3k-activity-event","event_type":"biochemical_relationship","label":"Another downstream insulin-signaling step became more active.","description":"Chromium picolinate increased insulin-stimulated PI3K activity in muscle from the obese rats without increasing PI3K protein abundance.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"89d5d39c-2663-5e0c-8eae-562a5b25a47d","slug":"chromium-iii-picolinate","display_name":"Chromium(III) picolinate / CrPic","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fe056b29-565f-5535-a9cd-6e4e2c0d7c5f","slug":"rat-pi3k-complexes","display_name":"Rat phosphatidylinositol 3-kinase complexes (isoform unresolved)","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chromium-research/16424121.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b0fa1d0917ec9cf4f3d309f2e9eb0e2b498e083ed1ff881b8f8df94a3cbe8119\", \"start_char\": 0, \"end_char\": 1734, \"text_sha256\": \"b0fa1d0917ec9cf4f3d309f2e9eb0e2b498e083ed1ff881b8f8df94a3cbe8119\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Three-month supplementation and acute muscle insulin-signaling experiment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Chromium picolinate supplying approximately 80 µg Cr/kg/day; small obese and lean treatment groups","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Model-specific supplementation. Lower phosphatase protein and activity do not prove direct chromium binding to PTP1B. Different results from cultured adipocytes are contextual, not a reason to erase either study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chromium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chromium","display_name":"Chromium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Male JCR:LA-cp obese and lean rats","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Another downstream insulin-signaling step became more active.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chromium-p16424121] Chromium picolinate enhances skeletal muscle cellular insulin signaling in vivo in obese, insulin-resistant JCR:LA-cp rats. (2006). https://pubmed.ncbi.nlm.nih.gov/16424121/ DOI: 10.1093/jn/136.2.415","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Vastus lateralis skeletal muscle","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8e1cdd31-4589-5bb3-8cff-40e5da91892e","evidence_kind":"source_excerpt","locator":"Lines 432-443","start_line":432,"end_line":443,"excerpt":"### chromium-rat-pi3k-activity\nChromium picolinate increased insulin-stimulated PI3K activity in muscle from the obese rats without increasing PI3K protein abundance.\nCondition category: normal\nnutrient_topic: Chromium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Another downstream insulin-signaling step became more active.\norganism: Male JCR:LA-cp obese and lean rats\ntissue_or_cell_type: Vastus lateralis skeletal muscle\nexperimental_model: Three-month supplementation and acute muscle insulin-signaling experiment\nlimitations: Model-specific supplementation. Lower phosphatase protein and activity do not prove direct chromium binding to PTP1B. Different results from cultured adipocytes are contextual, not a reason to erase either study.\nexposure: Chromium picolinate supplying approximately 80 µg Cr/kg/day; small obese and lean treatment groups\nevidence_span: {\"source_cache\": \"artifacts/chromium-research/16424121.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b0fa1d0917ec9cf4f3d309f2e9eb0e2b498e083ed1ff881b8f8df94a3cbe8119\", \"start_char\": 0, \"end_char\": 1734, \"text_sha256\": \"b0fa1d0917ec9cf4f3d309f2e9eb0e2b498e083ed1ff881b8f8df94a3cbe8119\"}\n[chromium-p16424121] Chromium picolinate enhances skeletal muscle cellular insulin signaling in vivo in obese, insulin-resistant JCR:LA-cp rats. 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