{"id":"2d57863f-ac7a-5ca8-a87c-11c87cb1135a","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-mouse-slc19a3-absorption-loss","predicate":"reduces","statement":"Slc19a3-null mice had lower thiamine uptake in isolated intestinal cells and intact loops.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"31e4fbee-90d7-574e-964f-089b2d6f1b66","mechanism_event_label":"Loss of transporter 2 reduced intestinal B1 entry in mice.","subject":{"id":"26e44a2f-af09-5a32-9b4c-eab829cb1e89","slug":"mouse-slc19a3-null-genotype","display_name":"Mouse Slc19a3-null genotype","entity_type_key":"gene"},"object":{"id":"79bf8987-06cf-5d7c-8f8a-71fe7f9c2698","slug":"intestinal-thiamine-uptake","display_name":"Intestinal free-thiamine uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"31e4fbee-90d7-574e-964f-089b2d6f1b66","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-mouse-slc19a3-absorption-loss-event","event_type":"biochemical_relationship","label":"Loss of transporter 2 reduced intestinal B1 entry in mice.","description":"Slc19a3-null mice had lower thiamine uptake in isolated intestinal cells and intact loops.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d4c2b412-1eca-52bf-9334-6c855497abef","slug":"mouse-slc19a3","display_name":"Mouse thiamine transporter 2 / Slc19a3","entity_type_key":"protein"},"role":"deleted transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"26e44a2f-af09-5a32-9b4c-eab829cb1e89","slug":"mouse-slc19a3-null-genotype","display_name":"Mouse Slc19a3-null genotype","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"79bf8987-06cf-5d7c-8f8a-71fe7f9c2698","slug":"intestinal-thiamine-uptake","display_name":"Intestinal free-thiamine uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence-scope","value_text":"Intestine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_locator","value_text":"Abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_spans","value_text":"[{\"source_document\": \"artifacts/thiamine_transport_sources/reidling-2010-slc19a3-knockout-source-record.json\", \"source_field\": \"resultList.result[0].abstractText\", \"start_char\": 0, \"end_char\": 1719}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Slc19a3-null or Slc19a2-null mice versus littermates; cells, intestinal loops and expression assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cannot assign the same quantitative effect to humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Thiamine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Loss of transporter 2 reduced intestinal B1 entry in mice.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[reidling-2010-slc19a3-knockout] Impaired intestinal vitamin B1 (thiamin) uptake in thiamin transporter-2-deficient mice (2010). https://pubmed.ncbi.nlm.nih.gov/19879271/ DOI: 10.1053/j.gastro.2009.10.042","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Intestine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"57ca99e4-2fa6-5bdd-a2ef-fab60c3d03bf","evidence_kind":"source_excerpt","locator":"Lines 238-250","start_line":238,"end_line":250,"excerpt":"### b1-mouse-slc19a3-absorption-loss\nSlc19a3-null mice had lower thiamine uptake in isolated intestinal cells and intact loops.\nCondition category: machinery_impairment\nnutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Loss of transporter 2 reduced intestinal B1 entry in mice.\norganism: Mus musculus\ntissue_or_cell_type: Intestine\nexperimental_model: Slc19a3-null or Slc19a2-null mice versus littermates; cells, intestinal loops and expression assays.\nlimitations: Cannot assign the same quantitative effect to humans.\nevidence_spans: [{\"source_document\": \"artifacts/thiamine_transport_sources/reidling-2010-slc19a3-knockout-source-record.json\", \"source_field\": \"resultList.result[0].abstractText\", \"start_char\": 0, \"end_char\": 1719}]\nevidence_locator: Abstract\nevidence-scope: Intestine\n[reidling-2010-slc19a3-knockout] Impaired intestinal vitamin B1 (thiamin) uptake in thiamin transporter-2-deficient mice (2010). https://pubmed.ncbi.nlm.nih.gov/19879271/ DOI: 10.1053/j.gastro.2009.10.042","model_system":"Slc19a3-null or Slc19a2-null mice versus littermates; cells, intestinal loops and expression assays.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [reidling-2010-slc19a3-knockout] Impaired intestinal vitamin B1 (thiamin) uptake in thiamin transporter-2-deficient mice (2010). https://pubmed.ncbi.nlm.nih.gov/19879271/ DOI: 10.1053/j.gastro.2009.10.042","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"158d2c03-ac8c-589f-8270-c468165ae346","stable_key":"import-46d15d9e-d3b5-544d-ba01-b785aa3e4f42","title":"Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f376512fb3310141315548015b387833e3af45146e73fb73cd02cee20e4ddb9c","revision_id":"53bc5eda-dec8-58cc-a56f-a9eb4ab036ef","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}