{"id":"2cf54762-a4d9-5ef3-b59d-c2227ecc291c","stable_key":"e3f6ce02-0bd7-5103-acc1-68503ab6751b:drg-p65","predicate":"increases_in_recorded_comparison","statement":"ALCAR increased p65/RelA acetylation in the DRG culture study.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"fe59ef93-49ec-50f4-8918-d0982964abb7","mechanism_event_label":"ALCAR increased p65/RelA acetylation in the DRG culture study.","subject":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"object":{"id":"c3ad9c2d-2fc1-591f-b512-688b8758b21d","slug":"rat-drg-p65-acetylation","display_name":"Rat DRG p65/RelA acetylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"fe59ef93-49ec-50f4-8918-d0982964abb7","stable_key":"e3f6ce02-0bd7-5103-acc1-68503ab6751b:drg-p65-event","event_type":"experimental_observation","label":"ALCAR increased p65/RelA acetylation in the DRG culture study.","description":"### drg-p65\n\nALCAR increased p65/RelA acetylation in the DRG culture study.\nFree carnitine did not reproduce the p65 acetylation or Grm2 mRNA changes. This comparison does not establish acetate equivalence or a universal carnitine/ALCAR distinction in every cell.\nModel and exposure: Rat primary DRG cultures; 250 micromolar exposure.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Transcriptional regulation of metabotropic glutamate receptor 2/3 expression by the NF-kappaB pathway in primary dorsal root ganglia neurons: a possible mechanism for the analgesic effect of L-acetylcarnitine. | 2006 | https://pubmed.ncbi.nlm.nih.gov/16764720/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC1550235/ | https://doi.org/10.1186/1744-8069-2-20 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"ALCAR exposure","sequence_order":0,"notes":""},{"entity":{"id":"c3ad9c2d-2fc1-591f-b512-688b8758b21d","slug":"rat-drg-p65-acetylation","display_name":"Rat DRG p65/RelA acetylation","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""},{"entity":{"id":"f6a4422d-82bc-5979-b1d9-54984971b126","slug":"rat-rela","display_name":"Rat NF-kappaB p65 / Rela","entity_type_key":"protein"},"role":"measured acetylated protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"negative comparator","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"ALCAR exposure\", \"comparator\": \"Vehicle\", \"endpoint\": \"ALCAR increased p65/RelA acetylation in the DRG culture study.\", \"effect_direction\": \"increase\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Rat primary DRG cultures; 250 micromolar exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived curation with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Acetyl-L-carnitine collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"ALCAR increased p65/RelA acetylation in the DRG culture study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Transcriptional regulation of metabotropic glutamate receptor 2/3 expression by the NF-kappaB pathway in primary dorsal root ganglia neurons: a possible mechanism for the analgesic effect of L-acetylcarnitine. | https://pubmed.ncbi.nlm.nih.gov/16764720/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC1550235/ | https://doi.org/10.1186/1744-8069-2-20","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference drg-p65; Free carnitine did not reproduce the p65 acetylation or Grm2 mRNA changes. This comparison does not establish acetate equivalence or a universal carnitine/ALCAR distinction in every cell.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ff13c9ff-9ca8-5ac0-a35a-b2490ebfe6c5","evidence_kind":"source_excerpt","locator":"Lines 208-214","start_line":208,"end_line":214,"excerpt":"### drg-p65\n\nALCAR increased p65/RelA acetylation in the DRG culture study.\nFree carnitine did not reproduce the p65 acetylation or Grm2 mRNA changes. This comparison does not establish acetate equivalence or a universal carnitine/ALCAR distinction in every cell.\nModel and exposure: Rat primary DRG cultures; 250 micromolar exposure.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Transcriptional regulation of metabotropic glutamate receptor 2/3 expression by the NF-kappaB pathway in primary dorsal root ganglia neurons: a possible mechanism for the analgesic effect of L-acetylcarnitine. | 2006 | https://pubmed.ncbi.nlm.nih.gov/16764720/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC1550235/ | https://doi.org/10.1186/1744-8069-2-20 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"Rat primary DRG cultures; 250 micromolar exposure.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2588f904-245a-597a-8a12-65118013fb90","stable_key":"import-e3f6ce02-0bd7-5103-acc1-68503ab6751b","title":"Acetyl-L-carnitine: detailed mechanisms of action (reviewed 6 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original review of primary studies and clearly identified official records. Exact primary access is stated; no independent replication is claimed.","file_path":"","sha256":"84cc82d6fbb0fd5d73c4ce779e9f88e7f0184ba2d29b18ad0f153815380f2975","revision_id":"dac7a950-dbe5-5c7a-9c70-2ea711d5c71a","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}