{"id":"2ca01ff8-aaf0-5a69-9bb7-22003ba12f68","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-plc-location","predicate":"increases_membrane_abundance","statement":"Adding t10,c12 CLA increased plasma-membrane PLC-gamma1 abundance within three minutes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"72a637a3-faba-5746-8cc0-281b9fc90ab2","mechanism_event_label":"A lipid-signaling enzyme moved toward the membrane.","subject":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"object":{"id":"4b863610-cd78-5f40-a114-e4c8b7b7d96e","slug":"plcg1","display_name":"PLCG1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"72a637a3-faba-5746-8cc0-281b9fc90ab2","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-plc-location-event","event_type":"observed_relationship","label":"A lipid-signaling enzyme moved toward the membrane.","description":"Adding t10,c12 CLA increased plasma-membrane PLC-gamma1 abundance within three minutes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4b863610-cd78-5f40-a114-e4c8b7b7d96e","slug":"plcg1","display_name":"PLCG1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary human adipocyte localization experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Localization is not proof of direct CLA binding or identification of its receptor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A lipid-signaling enzyme moved toward the membrane.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The phospholipase C inhibitor U73122 attenuates trans-10, cis-12 conjugated linoleic acid-mediated inflammatory signaling and insulin resistance in human adipocytes. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23468551/ · DOI 10.3945/jn.112.173161","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e4eaf12a-38fe-5ea9-8d55-8b19acaf568a","evidence_kind":"source_excerpt","locator":"Lines 166-172","start_line":166,"end_line":172,"excerpt":"## cla-plc-location\nA lipid-signaling enzyme moved toward the membrane.\nAdding t10,c12 CLA increased plasma-membrane PLC-gamma1 abundance within three minutes.\nModel: Primary human adipocyte localization experiment.\nLimitations: Localization is not proof of direct CLA binding or identification of its receptor.\nEvidence access: Primary abstract\nThe phospholipase C inhibitor U73122 attenuates trans-10, cis-12 conjugated linoleic acid-mediated inflammatory signaling and insulin resistance in human adipocytes. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23468551/ · DOI 10.3945/jn.112.173161","model_system":"Primary human adipocyte localization experiment.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f9fe73a8a4008d52061c65abe6de7a9806da75f1c478c5adace9387b8ffaad9e","revision_id":"21196d9c-aaab-5fe1-8f32-2987c94289c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}